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GFAP Polyclonal Antibody (A0237)

Reviews (2)Publications (2) Datasheet

ABclonal:Western blot - GFAP Polyclonal Antibody (A0237)
ABclonal:Immunofluorescence - GFAP Polyclonal Antibody (A0237)
Review
ABclonal:Immunofluorescence (IF) review for GFAP Polyclonal Antibody(A0237)
Review
ABclonal:Immunohistochemistry (IHC) review for GFAP Polyclonal Antibody(A0237)

Overview

Product nameGFAP Polyclonal Antibody
Catalog No.A0237
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 300 to the C-terminus of human GFAP (NP_002046.1).
SequenceRGTNESLERQMREQEERHVREAASYQEALARLEEEGQSLKDEMARHLQEYQDLLNVKLALDIEIATYRKLLEGEENRITIPVQTFSNLQIRETSLDTKSVSEGHLKRNIVVKTVEMRDGEVIKESKQEHKDVM
Gene ID2670
Swiss protP14136
SynonymsGFAP; ALXDRD; glial fibrillary acidic protein
Calculated MW49kDa/50kDa
Observed MW49kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesPC-12, Mouse brain
Cellular locationCytoplasm
Customer validation

IF(Mouse glial cells, Human)

IHC(Mouse brain)

WB(Mouse)

IF(Mouse)

ABclonal:Western blot - GFAP Polyclonal Antibody (A0237) }

Western blot - GFAP Polyclonal Antibody (A0237)

Western blot analysis of extracts of various cell lines, using GFAP antibody (A0237) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - GFAP Polyclonal Antibody (A0237) }

Immunofluorescence - GFAP Polyclonal Antibody (A0237)

Immunofluorescence analysis of U-251MG cells using GFAP antibody (A0237) at dilution of 1:100. Blue: DAPI for nuclear staining.

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