Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

GFAP Mouse mAb (A0014)

Publications (3) Datasheet SDS

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - GFAP Mouse mAb (A0014)

Western blot analysis of lysates from MCF-7 cells, using GFAP Mouse mAb (A0014).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Overview

Product name GFAP Mouse mAb
Catalog No. A0014
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes one of the major intermediate filament proteins of mature astrocytes. It is used as a marker to distinguish astrocytes from other glial cells during development. Mutations in this gene cause Alexander disease, a rare disorder of astrocytes in the central nervous system. Alternative splicing results in multiple transcript variants encoding distinct isoforms.

Immunogen information

Immunogen Recombinant protein of human GFAP
Sequence Email for sequence
Gene ID 2670
Swiss prot P14136
Synonyms ALXDRD; GFAP
Calculated MW 50kDa
Observed MW 50kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:100
  • IF/ICC 1:20 - 1:50
Storage buffer Store at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples MCF7
Cellular location Cytoplasm
Customer validation

WB (Rattus norvegicus)

IF (Mus musculus, Rattus norvegicus)

Research Area

GFAP Mouse mAb images

ABclonal:Western blot - GFAP Mouse mAb (A0014)}

Western blot - GFAP Mouse mAb (A0014)

Western blot analysis of lysates from MCF-7 cells, using GFAP Mouse mAb (A0014).
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

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Related Targets

Based on existing publications, the following genes are closely related to research on GFAP. (Numbers indicate number of published articles where both targets appear.)

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Related Research Area

Based on existing publications, the following research topics are related to GFAP. (Distance between topics and target gene indicate popularity.) GFAP

* Data provided by citexs.com, for reference only.

Publishing research using A0014? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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