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GCLM Rabbit pAb (A5314)

Reviews (2)Publications (4) Datasheet

ABclonal:Western blot - GCLM Rabbit pAb (A5314)

Western blot analysis of extracts of various cell lines, using GCLM antibody (A5314) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunofluorescence - GCLM Rabbit pAb (A5314)

Immunofluorescence analysis of U2OS cells using GCLM antibody (A5314). Blue: DAPI for nuclear staining.

Review
ABclonal: review for GCLM Rabbit pAb(A5314)
Review
ABclonal: review for GCLM Rabbit pAb(A5314)

Overview

Product nameGCLM Rabbit pAb
Catalog No.A5314
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).
SequenceMGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
Gene ID2730
Swiss protP48507
SynonymsGCLM; GLCLR
Calculated MW28kDa/30kDa
Observed MW31kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, Mouse liver, Mouse spleen, Mouse kidney, Rat kidney
Cellular location
Customer validation

(unknow, Mouse liver)

WB(Mus musculus, Homo sapiens)

ABclonal:Western blot - GCLM Rabbit pAb (A5314) }

Western blot - GCLM Rabbit pAb (A5314)

Western blot analysis of extracts of various cell lines, using GCLM antibody (A5314) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunofluorescence - GCLM Rabbit pAb (A5314) }

Immunofluorescence - GCLM Rabbit pAb (A5314)

Immunofluorescence analysis of U2OS cells using GCLM antibody (A5314). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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