Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

GCLM Rabbit pAb (A13989)

Publications (3) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:Western blot - GCLM Rabbit pAb (A13989)

Western blot analysis of extracts of various cell lines, using GCLM antibody (A13989) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

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Overview

Product name GCLM Rabbit pAb
Catalog No. A13989
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase, is the first rate limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. Gamma glutamylcysteine synthetase deficiency has been implicated in some forms of hemolytic anemia. Alternative splicing results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-274 of human GCLM (NP_002052.1).
Sequence MGTDSRAAKALLARARTLHLQTGNLLNWGRLRKKCPSTHSEELHDCIQKTLNEWSSQINPDLVREFPDVLECTVSHAVEKINPDEREEMKVSAKLFIVESNSSSSTRSAVDMACSVLGVAQLDSVIIASPPIEDGVNLSLEHLQPYWEELENLVQSKKIVAIGTSDLDKTQLEQLYQWAQVKPNSNQVNLASCCVMPPDLTAFAKQFDIQLLTHNDPKELLSEASFQEALQESIPDIQAHEWVPLWLLRYSVIVKSRGIIKSKGYILQAKRRGS
Gene ID 2730
Swiss prot P48507
Synonyms GLCLR; GCLM
Calculated MW 31kDa
Observed MW 31kDa

Applications

Reactivity Human, Mouse
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples H460, HT-29, K562, ES2, U-937, 22Rv1, U-251MG, mouse liver
Cellular location cytosol, glutamate-cysteine ligase complex
Customer validation

WB (Mus musculus, Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

GCLM Rabbit pAb images

ABclonal:Western blot - GCLM Rabbit pAb (A13989)}

Western blot - GCLM Rabbit pAb (A13989)

Western blot analysis of extracts of various cell lines, using GCLM antibody (A13989) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).

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Related Targets

Based on existing publications, the following genes are closely related to research on GCLM. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GCLM. (Distance between topics and target gene indicate popularity.) GCLM

* Data provided by citexs.com, for reference only.

Publishing research using A13989? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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