Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

GATA3 Rabbit mAb (A19636)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse

ABclonal:ChIP-seq - GATA3 Rabbit mAb (A19636)

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (A19636). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.

ABclonal:ChIP-seq - GATA3 Rabbit mAb (A19636)

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (A19636). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.

ABclonal:Western blot - GATA3 Rabbit mAb (A19636)

Western blot analysis of various lysates using GATA3 Rabbit mAb (A19636) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunoprecipitation - GATA3 Rabbit mAb (A19636)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg GATA3 antibody (A19636). Western blot was performed from the immunoprecipitate using GATA3 antibody (A19636) at a dilution of 1:500.

ABclonal:Chromatin Immunoprecipitation - GATA3 Rabbit mAb (A19636)

Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 Rabbit mAb antibody (A19636) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Overview

Product name GATA3 Rabbit mAb
Catalog No. A19636
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0115

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein contains two GATA-type zinc fingers and is an important regulator of T-cell development and plays an important role in endothelial cell biology. Defects in this gene are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human GATA3 (P23771).
Sequence MEVTADQPRWVSHHHPAVLNGQHPDTHHPGLSHSYMDAAQYPLPEEVDVLFNIDGQGNHVPPYYGNSVRATVQRYPPTHHGSQVCRPPLLHGSLPWLDGGKALGSHHTASPWNLSPFSKTSIHHGSPGPLSVYPPASSSSLSGGHASPHLFTFPPTPPKDVSPDPSLSTPGSAGSARQDEKECLKYQVPLPDSMKLESSH
Gene ID 2625
Swiss prot P23771
Synonyms HDR; HDRS; GATA3
Calculated MW 48kDa
Observed MW 48kDa

Applications

Reactivity Human, Mouse
Tested applications Testing results
WB Human
Recommended dilution
  • WB 1:500 - 1:2000
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ChIP 5μg antibody for 10μg-15μg of Chromatin
  • ChIP-seq 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunoprecipitation    
Positive samples SH-SY5Y, MCF7
Cellular location Nucleus
Customer validation

WB (Homo sapiens, Mus musculus)

IHC (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

GATA3 Rabbit mAb images

ABclonal:ChIP-seq - GATA3 Rabbit mAb (A19636)}

ChIP-seq - GATA3 Rabbit mAb (A19636)

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (A19636). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.
ABclonal:ChIP-seq - GATA3 Rabbit mAb (A19636)}

ChIP-seq - GATA3 Rabbit mAb (A19636)

Chromatin immunoprecipitations were performed with cross-linked chromatin from 293T cells transfected with GATA3 and GATA3 (A19636). The ChIP sequencing results indicate the enrichment pattern of GATA3 in selected genomic region and representative gene loci (SH3RF3), as shown in figure.
ABclonal:Western blot - GATA3 Rabbit mAb (A19636)}

Western blot - GATA3 Rabbit mAb (A19636)

Western blot analysis of various lysates using GATA3 Rabbit mAb (A19636) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunoprecipitation - GATA3 Rabbit mAb (A19636)}

Immunoprecipitation - GATA3 Rabbit mAb (A19636)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg GATA3 antibody (A19636). Western blot was performed from the immunoprecipitate using GATA3 antibody (A19636) at a dilution of 1:500.
ABclonal:Chromatin Immunoprecipitation - GATA3 Rabbit mAb (A19636)}

Chromatin Immunoprecipitation - GATA3 Rabbit mAb (A19636)

Chromatin immunoprecipitation analysis of extracts of MCF7 cells, using GATA3 Rabbit mAb antibody (A19636) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Related Targets

Based on existing publications, the following genes are closely related to research on GATA3. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GATA3. (Distance between topics and target gene indicate popularity.) GATA3

* Data provided by citexs.com, for reference only.

Publishing research using A19636? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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