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GATA1 Rabbit mAb (A21262)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human

ABclonal:Western blot - GATA1 Rabbit mAb (A21262)

Western blot analysis of extracts of various cell lines, using GATA1 antibody (A21262) at 1:11000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameGATA1 Rabbit mAb
Catalog No.A21262
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.
ImmunogenRecombinant protein of human GATA1
SequenceEmail for sequence
Gene ID2623
Swiss protP15976
SynonymsGATA1; ERYF1; GATA-1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT; GATA binding protein 1
Calculated MW34kDa/35kDa/42kDa
Observed MW47KDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHEL, HeLa
Cellular locationNucleus
ABclonal:Western blot - GATA1 Rabbit mAb (A21262)}

Western blot - GATA1 Rabbit mAb (A21262)

Western blot analysis of extracts of various cell lines, using GATA1 antibody (A21262) at 1:11000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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