Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

GATA1 Rabbit mAb (A21262)

Datasheet SDS COA

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human

ABclonal:Western blot - GATA1 Rabbit mAb (A21262)

Western blot analysis of various lysates, using GATA1 antibody (A21262) at 1:1600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product nameGATA1 Rabbit mAb
Catalog No.A21262
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC53588

Background

This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.

Immunogen information

ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GATA1 (NP_002040.1).
SequenceMEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGK
Gene ID2623
Swiss protP15976
SynonymsGATA1; ERYF1; GATA-1; GF-1; GF1; NF-E1; NFE1; XLANP; XLTDA; XLTT; GATA binding protein 1
Calculated MW34kDa/35kDa/42kDa
Observed MW47KDa

Applications

ReactivityHuman
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:1000 - 1:1600
  • IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunoprecipitation    
Positive samplesHEL, HeLa(negative)
Cellular locationNucleus

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

GATA1 Rabbit mAb images

ABclonal:Western blot - GATA1 Rabbit mAb (A21262)}

Western blot - GATA1 Rabbit mAb (A21262)

Western blot analysis of various lysates, using GATA1 antibody (A21262) at 1:1600 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Inquire About This Product

Submit your question about A21262 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order