Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

GATA1 Rabbit mAb (A21262)

Datasheet SDS COA

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPFCMCUT&TagmeRIPInhibition

Reactivity:Human

ABclonal:Western blot - GATA1 Rabbit mAb (A21262)

Western blot analysis of various lysates, using GATA1 antibody (A21262) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

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    Overview

    Product nameGATA1 Rabbit mAb
    Catalog No.A21262
    Host speciesRabbit
    Purification methodAffinity purification
    IsotypeIgG
    CloneNo.ARC53588

    Background

    This gene encodes a protein which belongs to the GATA family of transcription factors. The protein plays an important role in erythroid development by regulating the switch of fetal hemoglobin to adult hemoglobin. Mutations in this gene have been associated with X-linked dyserythropoietic anemia and thrombocytopenia.

    Immunogen information

    ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GATA1 (NP_002040.1).
    SequenceMEFPGLGSLGTSEPLPQFVDPALVSSTPESGVFFPSGPEGLDAAASSTAPSTATAAAAALAYYRDAEAYRHSPVFQVYPLLNCMEGIPGGSPYAGWAYGK
    Gene ID2623
    Swiss protP15976
    SynonymsGF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1; HAEADA
    Calculated MW43kDa
    Observed MW47kDa

    Applications

    ReactivityHuman
    Tested applicationsTesting results
    WBHuman
    Recommended dilution
    • WB 1:000 - 1:5000
    • IP 1:50 - 1:200
    Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
    Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
    Application keyWestern blotting    Immunoprecipitation    
    Positive samplesHEL
    Cellular locationNucleus

    Documents

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    Lot number

    Research Area

    GATA1 Rabbit mAb images

    ABclonal:Western blot - GATA1 Rabbit mAb (A21262)}

    Western blot - GATA1 Rabbit mAb (A21262)

    Western blot analysis of various lysates, using GATA1 antibody (A21262) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 3s.

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    Related Targets

    Based on existing publications, the following genes are closely related to research on GATA1. (Numbers indicate number of published articles where both targets appear.)

    1/

    Related Research Area

    Based on existing publications, the following research topics are related to GATA1. (Distance between topics and target gene indicate popularity.) GATA1

    * Data provided by citexs.com, for reference only.

    Publishing research using A21262? Please let us know so that we can cite the reference in this datasheet.

    * For research use only. Not for therapeutic or diagnostic purposes.

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