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GAPDH Rabbit pAb (AC001)

Review (1)Publications (85) Datasheet

ABclonal:GAPDH Rabbit pAb
ABclonal:Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC001) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry of paraffin-embedded human colon using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of C6 cells using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for GAPDH Rabbit pAb(AC001)

Overview

Product nameGAPDH Rabbit pAb
Catalog No.AC001
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID2597
Swiss protP04406
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW31kDa/36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:10000
IHC 1:50 - 1:100
IF 1:20 - 1:50
IP 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, HepG2, Mouse liver, Mouse spleen, Mouse kidney, Rat liver, Rat spleen, Rat kidney
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

(MSC cells)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Sus scrofa, chicken eggs)

ELISA(Mus musculus)

Co-IP(Arabidopsis thaliana)

ABclonal:Western blot - GAPDH Rabbit pAb (AC001) }

Western blot - GAPDH Rabbit pAb (AC001)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC001) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - GAPDH Rabbit pAb (AC001) }

Immunohistochemistry - GAPDH Rabbit pAb (AC001)

Immunohistochemistry of paraffin-embedded human colon using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001) }

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of C6 cells using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001) }

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Rabbit pAb (AC001) }

Immunofluorescence - GAPDH Rabbit pAb (AC001)

Immunofluorescence analysis of U-2 OS cells using GAPDH Rabbit pAb (AC001) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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