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GAPDH Polyclonal Antibody (AC001)

Publications (10) Datasheet

ABclonal:Western blot - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)
ABclonal:Immunofluorescence - GAPDH antibody (AC001)

Overview

Product nameGAPDH Polyclonal Antibody
Catalog No.AC001
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID2597
Swiss protP04406
SynonymsGAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase
Calculated MW31kDa/36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:1000 - 1:10000
IHC 1:50 - 1:100
IF 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesPC-3, HepG2, 293T, HeLa, Mouse brain, Mouse heart, Mouse skeletal muscle
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Human, Mouse, Rat)

ABclonal:Western blot - GAPDH antibody (AC001)}

Western blot - GAPDH antibody (AC001)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC001) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)}

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded rat pancreas using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)}

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded human breast cancer using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)}

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GAPDH antibody (AC001)}

Immunohistochemistry - GAPDH antibody (AC001)

Immunohistochemistry of paraffin-embedded mouse heart using GAPDH antibody (AC001) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH antibody (AC001)}

Immunofluorescence - GAPDH antibody (AC001)

Immunofluorescence analysis of MCF-7 cells using GAPDH antibody (AC001). Blue: DAPI for nuclear staining.

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