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GAPDH Mouse mAb (High Dilution) (AC033)

Publications (81) Datasheet

ABclonal:GAPDH Mouse mAb (High Dilution)
ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody (AC033) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded Human esophageal using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH Mouse mAb (High Dilution)
Catalog No.AC033
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Note:Dilution method
Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
ImmunogenRecombinant protein of human GAPDH
SequenceEmail for sequence
Gene ID2597
Swiss protP04406
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW31kDa/36kDa
Observed MW36KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:50000 - 1:200000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Antibody Dilute ProcedurePut 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, 293T, Mouse liver, Mouse kidney, Mouse spleen, Rat liver, Rat kidney
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Other, Schisandra chinensis)

DB(Homo sapiens)

IP(Mus musculus)

IF(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033) }

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of various cell lines, using GAPDH Monoclonal Antibody (AC033) at 1:20000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033) }

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded Human esophageal using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033) }

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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