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GAPDH Mouse mAb (AC002)

Publications (224) Datasheet

ABclonal:GAPDH Mouse mAb
ABclonal:Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC002) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded rat kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH Mouse mAb
Catalog No.AC002
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human GAPDH
SequenceEmail for sequence
Gene ID2597
Swiss protP04406
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW31kDa/36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:5000 - 1:20000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, MCF7, Mouse liver, Mouse heart, Mouse brain, Rat heart, Rat brain
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa, Danio rerio, Ctenopharyngodon idellus, Gallus gallus, Fenneropenaeus chinensis, Nicotiana tabacum L., Yeast, Zika virus, Other, Chlorocebus sabaeus, Giardia duodenalis, Capra hircus)

IF(Homo sapiens, Mus musculus)

IP(Homo sapiens)

WB(Homo sapiens)

IHC(Mus musculus)

ELISA(Ctenopharyngodon idellus)

Co-IP(Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - GAPDH Mouse mAb (AC002) }

Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC002) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002) }

Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded rat kidney using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002) }

Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of C6 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002) }

Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of NIH-3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002) }

Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of U-2 OS cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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