Products > Catalog Antibodies > Tag and Loading Control Antibodies

GAPDH Mouse mAb (AC002)

Publications (396) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:GAPDH Mouse mAb
ABclonal:Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC002) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded human liver cancer using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded mouse stomach using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded rat lung using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH Mouse mAb
Catalog No.AC002
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (P04406).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID2597
Swiss protP04406
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW31kDa/36kDa
Observed MW36KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:5000 - 1:20000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, 293T, HepG2, Mouse liver, Mouse spleen, Rat liver, Rat kidney
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Mus musculus, Homo sapiens, Sus scrofa, Rattus norvegicus, Ctenopharyngodon idellus, Danio rerio, Nicotiana tabacum L., Gallus gallus, Fenneropenaeus chinensis, Yeast, Giardia duodenalis, Zika virus, Other, Chlorocebus sabaeus, Capra hircus, Arabidopsis thaliana, fenugreek, Gynura procumbens, Rhinopithecus, Hamster)

IF(Homo sapiens, Mus musculus)

ELISA(Ctenopharyngodon idellus)

IP(Homo sapiens)

WB(Homo sapiens)

IHC(Mus musculus)

IF(Mus musculus)

Co-IP(Homo sapiens)

Co-IP(Homo sapiens)

IP(Homo sapiens)

IP(Mus musculus)

ABclonal:Western blot - GAPDH Mouse mAb (AC002)}

Western blot - GAPDH Mouse mAb (AC002)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC002) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)}

Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded human liver cancer using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)}

Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded mouse stomach using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (AC002)}

Immunohistochemistry - GAPDH Mouse mAb (AC002)

Immunohistochemistry of paraffin-embedded rat lung using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (AC002)}

Immunofluorescence - GAPDH Mouse mAb (AC002)

Immunofluorescence analysis of NIH/3T3 cells using GAPDH Mouse mAb (AC002) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Inquire About This Product

Submit your question about AC002 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using AC002? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Add to Cart

or Request quote for bulk purchase

Contact us to order