Product Type > Antibodies > Tag and Loading Control Antibodies

GAPDH (Human Specific) Rabbit mAb (AC036)

Publications (17) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036)

Western blot analysis of lysates from HeLa cells, using GAPDH (Human Specific) Rabbit mAb (AC036) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036)

Western blot analysis of various lysates using GAPDH (Human Specific) Rabbit mAb (AC036) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GAPDH (Human Specific)  Rabbit mAb (AC036)

Immunofluorescence analysis of U-2 OS cells using GAPDH (Human Specific) Rabbit mAb (AC036) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name GAPDH (Human Specific) Rabbit mAb
Catalog No. AC036
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC5136-01

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (NP_002037.2).
Sequence MGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID 2597
Swiss prot P04406
Synonyms G3PD; GAPD; HEL-S-162eP; GAPDH (Human Specific)
Calculated MW 36kDa
Observed MW 36kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:50000 - 1:200000
  • IF/ICC 1:200 - 1:2000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, NCI-H460, HepG2
Cellular location Cytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB (Homo sapiens, Mus musculus, Rattus norvegicus)

IHC (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

GAPDH (Human Specific) Rabbit mAb images

ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036)}

Western blot - GAPDH (Human Specific) Rabbit mAb (AC036)

Western blot analysis of lysates from HeLa cells, using GAPDH (Human Specific) Rabbit mAb (AC036) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036)}

Western blot - GAPDH (Human Specific) Rabbit mAb (AC036)

Western blot analysis of various lysates using GAPDH (Human Specific) Rabbit mAb (AC036) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - GAPDH (Human Specific)  Rabbit mAb (AC036)}

Immunofluorescence - GAPDH (Human Specific) Rabbit mAb (AC036)

Immunofluorescence analysis of U-2 OS cells using GAPDH (Human Specific) Rabbit mAb (AC036) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on GAPDH. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GAPDH. (Distance between topics and target gene indicate popularity.) GAPDH

* Data provided by citexs.com, for reference only.

Publishing research using AC036? Please let us know so that we can cite the reference in this datasheet.

Antibodies (7)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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