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GAPDH (Human Specific) Rabbit mAb (AC036)

Publications (6) Datasheet

ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036)

Western blot analysis of extracts of HeLa cells, using GAPDH (Human Specific) antibody (AC036) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036)

Western blot analysis of extracts of various cell lines, using GAPDH (Human Specific) antibody (AC036) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GAPDH (Human Specific)  Rabbit mAb (AC036)

Immunofluorescence analysis of U-2 OS cells using GAPDH (Human Specific) antibody (AC036) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH (Human Specific) Rabbit mAb
Catalog No.AC036
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human GAPDH (Human Specific)
SequenceEmail for sequence
Gene ID2597
Swiss protP04406
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW31kDa/36kDa
Observed MW36kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:50000 - 1:200000
IF 1:200 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesHeLa, NCI-H460, HepG2
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Homo sapiens, Mus musculus)

ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036) }

Western blot - GAPDH (Human Specific) Rabbit mAb (AC036)

Western blot analysis of extracts of HeLa cells, using GAPDH (Human Specific) antibody (AC036) at 1:10000-1:2560000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Western blot - GAPDH (Human Specific)  Rabbit mAb (AC036) }

Western blot - GAPDH (Human Specific) Rabbit mAb (AC036)

Western blot analysis of extracts of various cell lines, using GAPDH (Human Specific) antibody (AC036) at 1:100000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - GAPDH (Human Specific)  Rabbit mAb (AC036) }

Immunofluorescence - GAPDH (Human Specific) Rabbit mAb (AC036)

Immunofluorescence analysis of U-2 OS cells using GAPDH (Human Specific) antibody (AC036) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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