Product Type > Antibodies > Tag and Loading Control Antibodies

GAPDH Mouse mAb (High Dilution) (AC033)

Publications (148) Datasheet SDS

Tested applications:WBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:GAPDH Mouse mAb (High Dilution)
ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of various lysates, using GAPDH Mouse mAb(High Dilution) antibody (AC033) at 1:640000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 different dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded human kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded mouse kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of PC-12 cells using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAPDH Mouse mAb (High Dilution)
Catalog No.AC033
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG2b, Kappa
CloneNo.AMC0062

Background

This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
Note：Dilution method
Put 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.

Immunogen information

ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-335 of human GAPDH (P04406).
SequenceMGKVKVGVNGFGRIGRLVTRAAFNSGKVDIVAINDPFIDLNYMVYMFQYDSTHGKFHGTVKAENGKLVINGNPITIFQERDPSKIKWGDAGAEYVVESTGVFTTMEKAGAHLQGGAKRVIISAPSADAPMFVMGVNHEKYDNSLKIISNASCTTNCLAPLAKVIHDNFGIVEGLMTTVHAITATQKTVDGPSGKLWRDGRGALQNIIPASTGAAKAVGKVIPELNGKLTGMAFRVPTANVSVVDLTCRLEKPAKYDDIKKVVKQASEGPLKGILGYTEHQVVSSDFNSDTHSSTFDAGAGIALNDHFVKLISWYDNEFGYSNRVVDLMAHMASKE
Gene ID2597
Swiss protP04406
SynonymsG3PD; GAPD; HEL-S-162eP; GAPDH
Calculated MW31kDa/36kDa
Observed MW36KDa

Applications

ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:10000 - 1:760000
  • IHC 1:100 - 1:500
  • IF/ICC 1:50 - 1:200
Antibody Dilute ProcedurePut 5ul AC033 into 45 ul buffer(with 50% glycerol), diluted 1:10, 000 for using. The diluted antibody can be stored at -20℃ without aliquot.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHeLa, 293T, HepG2, Mouse kidney, Rat liver
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Homo sapiens, Mus musculus, Rattus norvegicus, Other, Schisandra chinensis, Arabidopsis thaliana, Saccharomyces cerevisiae, Mesocricetus auratus)

DB(Homo sapiens)

IP(Mus musculus, Homo sapiens)

IF(Homo sapiens, Mus musculus)

Co-IP(Homo sapiens)

ICH(Mus musculus)

WB(Mus musculus)

ELISA(Mus musculus)

Research Area

GAPDH Mouse mAb (High Dilution) images

ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)}

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of various lysates, using GAPDH Mouse mAb(High Dilution) antibody (AC033) at 1:640000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - GAPDH Mouse mAb (High Dilution) (AC033)}

Western blot - GAPDH Mouse mAb (High Dilution) (AC033)

Western blot analysis of extracts of HeLa cells, using GAPDH antibody (AC033) at 1:10000-1:2560000 different dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)}

Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded human kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)}

Immunohistochemistry - GAPDH Mouse mAb (High Dilution) (AC033)

Immunohistochemistry of paraffin-embedded mouse kidney using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:500 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)}

Immunofluorescence - GAPDH Mouse mAb (High Dilution) (AC033)

Immunofluorescence analysis of PC-12 cells using GAPDH Mouse mAb (High Dilution) (AC033) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on GAPDH. (Numbers indicate number of published articles where both targets appear.)

◀1/▶

Related Research Area

Based on existing publications, the following research topics are related to GAPDH. (Distance between topics and target gene indicate popularity.) GAPDH

* Data provided by citexs.com, for reference only.

Publishing research using AC033? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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