Products > Catalog Antibodies > Tag and Loading Control Antibodies

GAPDH Monoclonal Antibody (AC002)

Publications (50) Datasheet

ABclonal:Western blot - GAPDH antibody (AC002)

Overview

Product nameGAPDH Monoclonal Antibody
Catalog No.AC002
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the glyceraldehyde-3-phosphate dehydrogenase protein family. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. The product of this gene catalyzes an important energy-yielding step in carbohydrate metabolism, the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD). The encoded protein has additionally been identified to have uracil DNA glycosylase activity in the nucleus. Also, this protein contains a peptide that has antimicrobial activity against E. coli, P. aeruginosa, and C. albicans. Studies of a similar protein in mouse have assigned a variety of additional functions including nitrosylation of nuclear proteins, the regulation of mRNA stability, and acting as a transferrin receptor on the cell surface of macrophage. Many pseudogenes similar to this locus are present in the human genome. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human GAPDH
SequenceEmail for sequence
Gene ID2597
Swiss protP04406
SynonymsGAPDH; G3PD; GAPD; HEL-S-162eP; glyceraldehyde-3-phosphate dehydrogenase
Calculated MW31kDa/36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:5000 - 1:20000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, HepG2, MCF7, Mouse liver, Mouse heart, Mouse brain, Rat heart, Rat brain
Cellular locationCytoplasm, Membrane, Nucleus, cytoskeleton, cytosol, perinuclear region
Customer validation

WB(Human, Mouse, White shrimp, Pig, Rat, Human, Grass carp, Zebrafish, human, Rat)

IF(Human)

Elisa(grass carp)

ABclonal:Western blot - GAPDH antibody (AC002)}

Western blot - GAPDH antibody (AC002)

Western blot analysis of extracts of various cell lines, using GAPDH antibody (AC002) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Submit an review for AC002

  1. Please choose your application, enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Inquire About This Product

Submit your question about AC002 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using AC002? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order