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GAP43 Rabbit mAb (A19055)

Publications (3) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - GAP43 Rabbit mAb (A19055)

Western blot analysis of extracts of various cell lines, using GAP43 antibody (A19055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)

Immunofluorescence analysis of rat retina using GAP43 Rabbit mAb (A19055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAP43 Rabbit mAb
Catalog No.A19055
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 139-238 of human GAP43 (P17677).
SequenceETESATKASTDNSPSSKAEDAPAKEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETGESSQAEENIEAVDETKPKESARQDEGKEEEPEADQEHA
Gene ID2596
Swiss protP17677
SynonymsB-50; PP46; GAP43
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y, U-87MG, Mouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Cell projection, Cytoplasmic side, Peripheral membrane protein, filopodium membrane, growth cone membrane, synapse
Customer validation

IF(Rattus norvegicus)

WB(Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - GAP43 Rabbit mAb (A19055)}

Western blot - GAP43 Rabbit mAb (A19055)

Western blot analysis of extracts of various cell lines, using GAP43 antibody (A19055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)}

Immunofluorescence - GAP43 Rabbit mAb (A19055)

Immunofluorescence analysis of rat retina using GAP43 Rabbit mAb (A19055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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