Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

GAP43 Rabbit mAb (A19055)

Publications (4) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - GAP43 Rabbit mAb (A19055)

Western blot analysis of various lysates using GAP43 Rabbit mAb (A19055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Rat eye tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Rat brain tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Mouse eye tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Mouse brain tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

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Overview

Product name GAP43 Rabbit mAb
Catalog No. A19055
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC0246

Background

The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 139-238 of human GAP43 (P17677).
Sequence ETESATKASTDNSPSSKAEDAPAKEEPKQADVPAAVTAAAATTPAAEDAAAKATAQPPTETGESSQAEENIEAVDETKPKESARQDEGKEEEPEADQEHA
Gene ID 2596
Swiss prot P17677
Synonyms B-50; PP46; GAP-43; GAP43
Calculated MW 25kDa
Observed MW 43-48kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
WB HumanMouseRat
IF/ICC Rat
Recommended dilution
  • WB 1:500 - 1:2000
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples SH-SY5Y, U-87MG, Mouse brain, Rat brain
Cellular location Cell junction, Cell membrane, Cell projection, Cytoplasmic side, Peripheral membrane protein, filopodium membrane, growth cone membrane, synapse
Customer validation

IF (Rattus norvegicus)

WB (Rattus norvegicus, Homo sapiens)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

GAP43 Rabbit mAb images

ABclonal:Western blot - GAP43 Rabbit mAb (A19055)}

Western blot - GAP43 Rabbit mAb (A19055)

Western blot analysis of various lysates using GAP43 Rabbit mAb (A19055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)}

Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Rat eye tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)}

Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Rat brain tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)}

Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Mouse eye tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.
ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)}

Immunofluorescence - GAP43 Rabbit mAb (A19055)

Confocal imaging of paraffin-embedded Mouse brain tissue using GAP43 Rabbit mAb (A19055, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on GAP43. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to GAP43. (Distance between topics and target gene indicate popularity.) GAP43

* Data provided by citexs.com, for reference only.

Publishing research using A19055? Please let us know so that we can cite the reference in this datasheet.

Antibodies (3)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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