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GAP43 Rabbit mAb (A19055)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - GAP43 Rabbit mAb (A19055)

Western blot analysis of extracts of various cell lines, using GAP43 antibody (A19055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055)

Immunofluorescence analysis of rat retina using GAP43 Rabbit mAb (A19055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product nameGAP43 Rabbit mAb
Catalog No.A19055
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This protein is considered a crucial component of an effective regenerative response in the nervous system. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human GAP43
SequenceEmail for sequence
Gene ID2596
Swiss protP17677
SynonymsB-50; PP46; GAP43
Calculated MW48kDa
Observed MW48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesSH-SY5Y, U-87MG, Mouse brain, Rat brain
Cellular locationCell junction, Cell membrane, Cell projection, Cytoplasmic side, Peripheral membrane protein, filopodium membrane, growth cone membrane, synapse
ABclonal:Western blot - GAP43 Rabbit mAb (A19055) }

Western blot - GAP43 Rabbit mAb (A19055)

Western blot analysis of extracts of various cell lines, using GAP43 antibody (A19055) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunofluorescence - GAP43 Rabbit mAb (A19055) }

Immunofluorescence - GAP43 Rabbit mAb (A19055)

Immunofluorescence analysis of rat retina using GAP43 Rabbit mAb (A19055) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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