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GABARAP Rabbit pAb (A12568)

Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - GABARAP Rabbit pAb (A12568)

Western blot analysis of extracts of various cell lines, using GABARAP antibody (A12568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry of paraffin-embedded rat kidney using GABARAP antibody (A12568) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry of paraffin-embedded human liver using GABARAP antibody (A12568) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP antibody (A12568) at dilution of 1:100 (40x lens).

Overview

Product nameGABARAP Rabbit pAb
Catalog No.A12568
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Gamma-aminobutyric acid A receptors [GABA(A) receptors] are ligand-gated chloride channels that mediate inhibitory neurotransmission. This gene encodes GABA(A) receptor-associated protein, which is highly positively charged in its N-terminus and shares sequence similarity with light chain-3 of microtubule-associated proteins 1A and 1B. This protein clusters neurotransmitter receptors by mediating interaction with the cytoskeleton.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human GABARAP (NP_009209.1).
SequenceMKFVYKEEHPFEKRRSEGEKIRKKYPDRVPVIVEKAPKARIGDLDKKKYLVPSDLTVGQFYFLIRKRIHLRAEDALFFFVNNVIPPTSATMGQLYQEHHE
Gene ID11337
Swiss protO95166
SynonymsGABARAP; ATG8A; GABARAP-a; MM46
Calculated MW13kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesHepG2, Jurkat, HeLa
Cellular locationCytoplasm, Cytoplasmic vesicle, Endomembrane system, Golgi apparatus membrane, autophagosome, cytoskeleton
ABclonal:Western blot - GABARAP Rabbit pAb (A12568) }

Western blot - GABARAP Rabbit pAb (A12568)

Western blot analysis of extracts of various cell lines, using GABARAP antibody (A12568) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568) }

Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry of paraffin-embedded rat kidney using GABARAP antibody (A12568) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568) }

Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry of paraffin-embedded human liver using GABARAP antibody (A12568) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - GABARAP Rabbit pAb (A12568) }

Immunohistochemistry - GABARAP Rabbit pAb (A12568)

Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP antibody (A12568) at dilution of 1:100 (40x lens).

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