Western blot analysis of extracts of various cell lines, using GABARAPL2 Rabbit mAb (A9585) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
| Product name | GABARAPL2 Rabbit mAb |
|---|---|
| Catalog No. | A9585 |
| Host species | Rabbit |
| Purification method | Affinity purification |
| Isotype | IgG |
| Immunogen | A synthesized peptide derived from human GABARAPL2 |
|---|---|
| Sequence | Email for sequence |
| Gene ID | 11345 |
| Swiss prot | P60520 |
| Synonyms | ATG8; ATG8C; GATE-16; GATE16; GEF-2; GEF2 |
| Calculated MW | 14kDa |
| Observed MW | 16KDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP |
| Recommended dilution | WB 1:500 - 1:2000 |
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3. |
| Application key | Western blotting |
| Positive samples | PC-3, RD, Mouse brain, Rat brain |
| Cellular location |
* For research use only. Not for therapeutic or diagnostic purposes.
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