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GABARAPL2 Rabbit pAb (A7782)

Datasheet

ABclonal:Western blot - GABARAPL2 Polyclonal Antibody (A7782)
ABclonal:Immunohistochemistry - GABARAPL2 Polyclonal Antibody (A7782)
ABclonal:Immunohistochemistry - GABARAPL2 Polyclonal Antibody (A7782)
ABclonal:Immunofluorescence - GABARAPL2 Polyclonal Antibody (A7782)

Overview

Product nameGABARAPL2 Rabbit pAb
Catalog No.A7782
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL2 (NP_009216.1).
SequenceMKWMFKEDHSLEHRCVESAKIRAKYPDRVPVIVEKVSGSQIVDIDKRKYLVPSDITVAQFMWIIRKRIQLPSEKAIFLFVDKTVPQSSLTMGQLYEKEKDEDGFLYVAYSGENTFGF
Gene ID11345
Swiss protP60520
SynonymsGABARAPL2; ATG8; ATG8C; GATE-16; GATE16; GEF-2; GEF2
Calculated MW13kDa
Observed MW14kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:20 - 1:50
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMouse brain
Cellular locationCytoplasmic vesicle, Golgi apparatus, autophagosome
ABclonal:Western blot - GABARAPL2 Polyclonal Antibody (A7782) }

Western blot - GABARAPL2 Polyclonal Antibody (A7782)

Western blot analysis of extracts of mouse brain, using GABARAPL2 antibody (A7782) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunohistochemistry - GABARAPL2 Polyclonal Antibody (A7782) }

Immunohistochemistry - GABARAPL2 Polyclonal Antibody (A7782)

Immunohistochemistry of paraffin-embedded rat brain using GABARAPL2 antibody (A7782) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - GABARAPL2 Polyclonal Antibody (A7782) }

Immunohistochemistry - GABARAPL2 Polyclonal Antibody (A7782)

Immunohistochemistry of paraffin-embedded mouse heart using GABARAPL2 antibody (A7782) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - GABARAPL2 Polyclonal Antibody (A7782) }

Immunofluorescence - GABARAPL2 Polyclonal Antibody (A7782)

Immunofluorescence analysis of A549 cells using GABARAPL2 antibody (A7782). Blue: DAPI for nuclear staining.

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