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GABARAPL1 Rabbit pAb (A7790)

Datasheet

ABclonal:Western blot - GABARAPL1 Polyclonal Antibody (A7790)

Overview

Product nameGABARAPL1 Rabbit pAb
Catalog No.A7790
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-117 of human GABARAPL1 (NP_113600.1).
SequenceMKFQYKEDHPFEYRKKEGEKIRKKYPDRVPVIVEKAPKARVPDLDKRKYLVPSDLTVGQFYFLIRKRIHLRPEDALFFFVNNTIPPTSATMGQLYEDNHEEDYFLYVAYSDESVYGK
Gene ID23710
Swiss protQ9H0R8
SynonymsGABARAPL1; APG8-LIKE; APG8L; ATG8; ATG8B; ATG8L; GEC1
Calculated MW14kDa/17kDa
Observed MW16kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesBT-474, Mouse liver, Mouse kidney, Mouse brain, Rat brain, Rat kidney
Cellular locationCytoplasm, Cytoplasmic vesicle, Cytoplasmic vesicle membrane, Endoplasmic reticulum, Golgi apparatus, Lipid-anchor, autophagosome, cytoskeleton
ABclonal:Western blot - GABARAPL1 Polyclonal Antibody (A7790) }

Western blot - GABARAPL1 Polyclonal Antibody (A7790)

Western blot analysis of extracts of various cell lines, using GABARAPL1 antibody (A7790) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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