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G6PD Rabbit pAb (A1537)

Publications (2) Datasheet

ABclonal:Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of extracts of various cell lines, using G6PD antibody (A1537) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunofluorescence - G6PD Rabbit pAb (A1537)

Immunofluorescence analysis of HeLa cells using G6PD Rabbit pAb (A1537) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - G6PD Rabbit pAb (A1537)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug G6PD antibody (A1537). Western blot was performed from the immunoprecipitate using G6PD antibody (A1537) at a dilition of 1:2000.

Overview

Product nameG6PD Rabbit pAb
Catalog No.A1537
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes glucose-6-phosphate dehydrogenase. This protein is a cytosolic enzyme encoded by a housekeeping X-linked gene whose main function is to produce NADPH, a key electron donor in the defense against oxidizing agents and in reductive biosynthetic reactions. G6PD is remarkable for its genetic diversity. Many variants of G6PD, mostly produced from missense mutations, have been described with wide ranging levels of enzyme activity and associated clinical symptoms. G6PD deficiency may cause neonatal jaundice, acute hemolysis, or severe chronic non-spherocytic hemolytic anemia. Two transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 246-545 of human G6PD (NP_000393.4).
SequenceFANRIFGPIWNRDNIACVILTFKEPFGTEGRGGYFDEFGIIRDVMQNHLLQMLCLVAMEKPASTNSDDVRDEKVKVLKCISEVQANNVVLGQYVGNPDGEGEATKGYLDDPTVPRGSTTATFAAVVLYVENERWDGVPFILRCGKALNERKAEVRLQFHDVAGDIFHQQCKRNELVIRVQPNEAVYTKMMTKKPGMFFNPEESELDLTYGNRYKNVKLPDAYERLILDVFCGSQMHFVRSDELREAWRIFTPLLHQIELEKPKPIPYIYGSRGPTEADELMKRVGFQYEGTYKWVNPHKL
Gene ID2539
Swiss protP11413
SynonymsG6PD; G6PD1
Calculated MW59kDa/62kDa/63kDa
Observed MW60KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
IP 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, MCF7, A-431, C2C12, C6, Mouse thymus
Cellular location
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - G6PD Rabbit pAb (A1537) }

Western blot - G6PD Rabbit pAb (A1537)

Western blot analysis of extracts of various cell lines, using G6PD antibody (A1537) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Immunofluorescence - G6PD Rabbit pAb (A1537) }

Immunofluorescence - G6PD Rabbit pAb (A1537)

Immunofluorescence analysis of HeLa cells using G6PD Rabbit pAb (A1537) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - G6PD Rabbit pAb (A1537) }

Immunoprecipitation - G6PD Rabbit pAb (A1537)

Immunoprecipitation analysis of 300ug extracts of MCF7 cells using 3ug G6PD antibody (A1537). Western blot was performed from the immunoprecipitate using G6PD antibody (A1537) at a dilition of 1:2000.

* For research use only. Not for therapeutic or diagnostic purposes.

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