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Fibronectin Rabbit pAb (A12932)

Review (1)Publications (5) Datasheet

ABclonal:Western blot - Fibronectin Rabbit pAb (A12932)
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932)
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932)
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932)
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932)
Review
ABclonal: review for Fibronectin Rabbit pAb(A12932)

Overview

Product nameFibronectin Rabbit pAb
Catalog No.A12932
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. The encoded preproprotein is proteolytically processed to generate the mature protein. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants, at least one of which encodes an isoform that undergoes proteolytic processing. The full-length nature of some variants has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2200-2355 of human Fibronectin (NP_002017.1).
SequenceLLCQCLGFGSGHFRCDSSRWCHDNGVNYKIGEKWDRQGENGQMMSCTCLGNGKGEFKCDPHEATCYDDGKTYHVGEQWQKEYLGAICSCTCFGGQRGWRCDNCRRPGGEPSPEGTTGQSYNQYSQRYHQRTNTNVNCPIECFMPLDVQADREDSRE
Gene ID2335
Swiss protP02751
SynonymsFN1; CIG; ED-B; FINC; FN; FNZ; GFND; GFND2; LETS; MSF; fibronectin
Calculated MW71-73kDa/221-272kDa
Observed MW290kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesU-87MG, NIH/3T3
Cellular locationSecreted, extracellular matrix, extracellular space
Customer validation

(mouse cells)

WB(Homo sapiens,Mus musculus)

ICC(Homo sapiens)

ABclonal:Western blot - Fibronectin Rabbit pAb (A12932) }

Western blot - Fibronectin Rabbit pAb (A12932)

Western blot analysis of extracts of various cell lines, using Fibronectin antibody (A12932) at 1:3000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932) }

Immunohistochemistry - Fibronectin Rabbit pAb (A12932)

Immunohistochemistry of paraffin-embedded rat ovary using Fibronectin antibody (A12932) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932) }

Immunohistochemistry - Fibronectin Rabbit pAb (A12932)

Immunohistochemistry of paraffin-embedded human lung cancer using Fibronectin antibody (A12932) at dilution of 1:150 (40x lens).
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932) }

Immunohistochemistry - Fibronectin Rabbit pAb (A12932)

Immunohistochemistry of paraffin-embedded human liver cancer using Fibronectin antibody (A12932) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Fibronectin Rabbit pAb (A12932) }

Immunohistochemistry - Fibronectin Rabbit pAb (A12932)

Immunohistochemistry of paraffin-embedded mouse leydig cells using Fibronectin antibody (A12932) at dilution of 1:100 (40x lens).

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