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Ferritin Light Chain Rabbit mAb (A11241)

Datasheet

ABclonal:Western blot - Ferritin Light Chain Rabbit mAb (A11241)
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Overview

Product nameFerritin Light Chain Rabbit mAb
Catalog No.A11241
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human Ferritin Light Chain
SequenceEmail for sequence
Gene ID2512
Swiss protP02792
SynonymsLFTD; NBIA3
Calculated MW20kDa
Observed MW18-20KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse liver, Mouse spleen, Rat liver
Cellular location
ABclonal:Western blot - Ferritin Light Chain Rabbit mAb (A11241) }

Western blot - Ferritin Light Chain Rabbit mAb (A11241)

Western blot analysis of extracts of various cell lines, using Ferritin Light Chain Rabbit mAb (A11241) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241) }

Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry of paraffin-embedded rat brain using Ferritin Light Chain Rabbit mAb (A11241) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241) }

Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry of paraffin-embedded human esophageal using Ferritin Light Chain Rabbit mAb (A11241) at dilution of 1:100 (40x lens).

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