Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

Ferritin Light Chain Rabbit mAb (A11241)

Review (1) Publications (10) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - Ferritin Light Chain Rabbit mAb (A11241)

Western blot analysis of various lysates, using Ferritin Light Chain antibody (A11241) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Western blot - Ferritin Light Chain Rabbit mAb (A11241)

Western blot analysis of various lysates, using Ferritin Light Chain antibody (A11241) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human thyroid tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human tonsil tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded mouse spleen tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded rat kidney tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Review
ABclonal: review for Ferritin Light Chain Rabbit mAb(A11241)

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Overview

Product name Ferritin Light Chain Rabbit mAb
Catalog No. A11241
Host species Rabbit
Purification method Affinity purification
Isotype IgG
CloneNo. ARC57605

Background

This gene encodes the light subunit of the ferritin protein. Ferritin is the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in this light chain ferritin gene are associated with several neurodegenerative diseases and hyperferritinemia-cataract syndrome. This gene has multiple pseudogenes.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 1-175 of human Ferritin Light Chain (NP_000137.2).
Sequence MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD
Gene ID 2512
Swiss prot P02792
Synonyms LFTD; NBIA3; Ferritin Light Chain
Calculated MW 20kDa
Observed MW 18-20kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications Testing results
IHC-P HumanRat
WB HumanMouseRat
Recommended dilution
  • WB 1:2000 - 1:9000
  • IHC-P 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    
Positive samples Hep G2, 293T, Mouse ovary, Rat liver
Cellular location cytoplasm, cytosol, extracellular exosome, extracellular region
Customer validation

(293T、HeLa)

WB (Rattus norvegicus, Gallus gallus, Mus musculus, Homo sapiens)

IHC (Homo sapiens, Rattus norvegicus, Mus musculus, Homo sapiens Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Ferritin Light Chain Rabbit mAb images

ABclonal:Western blot - Ferritin Light Chain Rabbit mAb (A11241)}

Western blot - Ferritin Light Chain Rabbit mAb (A11241)

Western blot analysis of various lysates, using Ferritin Light Chain antibody (A11241) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Western blot - Ferritin Light Chain Rabbit mAb (A11241)}

Western blot - Ferritin Light Chain Rabbit mAb (A11241)

Western blot analysis of various lysates, using Ferritin Light Chain antibody (A11241) at 1:8000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)}

Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human thyroid tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)}

Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded human tonsil tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)}

Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded mouse spleen tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)}

Immunohistochemistry - Ferritin Light Chain Rabbit mAb (A11241)

Immunohistochemistry analysis of Ferritin Light Chain in paraffin-embedded rat kidney tissue using Ferritin Light Chain Rabbit mAb (A11241) at a dilution of 1:400 (40x lens).High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on FTL. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FTL. (Distance between topics and target gene indicate popularity.) FTL

* Data provided by citexs.com, for reference only.

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Antibodies (3)

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Secondary Antibodies (23)

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