Products > Catalog Antibodies > Polyclonal Antibodies

Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Reviews (2)Publications (3) Datasheet

ABclonal:Western blot - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase (FASN) antibody (A0461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunohistochemistry of paraffin-embedded human breast cancer using Fatty Acid Synthase (FASN) antibody (A0461) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunofluorescence analysis of A549 cells using Fatty Acid Synthase (FASN) antibody (A0461). Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunoprecipitation analysis of 600ug extracts of Mouse liver using 3ug Fatty Acid Synthase (FASN) antibody (A0461). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase (FASN) antibody (A0461) at a dilition of 1:1000.

Review
ABclonal: review for Fatty Acid Synthase (FASN) Rabbit pAb(A0461)
Review
ABclonal: review for Fatty Acid Synthase (FASN) Rabbit pAb(A0461)

Overview

Product nameFatty Acid Synthase (FASN) Rabbit pAb
Catalog No.A0461
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The enzyme encoded by this gene is a multifunctional protein. Its main function is to catalyze the synthesis of palmitate from acetyl-CoA and malonyl-CoA, in the presence of NADPH, into long-chain saturated fatty acids. In some cancer cell lines, this protein has been found to be fused with estrogen receptor-alpha (ER-alpha), in which the N-terminus of FAS is fused in-frame with the C-terminus of ER-alpha.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 2212-2511 of human Fatty Acid Synthase (FASN) (NP_004095.4).
SequenceQQQTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG
Gene ID2194
Swiss protP49327
SynonymsFAS; OA-519; SDR27X1; FASN
Calculated MW273kDa
Observed MW273kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesMouse liver, Mouse lung
Cellular locationCytoplasm, Melanosome
Customer validation

(Human fat cell, Breast cancer)

WB(Mus musculus)

ABclonal:Western blot - Fatty Acid Synthase (FASN) Rabbit pAb (A0461) }

Western blot - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Western blot analysis of extracts of various cell lines, using Fatty Acid Synthase (FASN) antibody (A0461) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - Fatty Acid Synthase (FASN) Rabbit pAb (A0461) }

Immunohistochemistry - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunohistochemistry of paraffin-embedded human breast cancer using Fatty Acid Synthase (FASN) antibody (A0461) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - Fatty Acid Synthase (FASN) Rabbit pAb (A0461) }

Immunofluorescence - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunofluorescence analysis of A549 cells using Fatty Acid Synthase (FASN) antibody (A0461). Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - Fatty Acid Synthase (FASN) Rabbit pAb (A0461) }

Immunoprecipitation - Fatty Acid Synthase (FASN) Rabbit pAb (A0461)

Immunoprecipitation analysis of 600ug extracts of Mouse liver using 3ug Fatty Acid Synthase (FASN) antibody (A0461). Western blot was performed from the immunoprecipitate using Fatty Acid Synthase (FASN) antibody (A0461) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0461

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A0461 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A0461? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order