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FasLG Rabbit pAb (A0234)

Review (1)Publications (7) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse

ABclonal:Western blot - FasLG Rabbit pAb (A0234)

Western blot analysis of extracts of Mouse heart, using FasLG antibody (A0234) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.

ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human esophageal cancer using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human tonsil using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human colon using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human placenta using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human lung cancer using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Review
ABclonal: review for FasLG Rabbit pAb(A0234)

Overview

Product nameFasLG Rabbit pAb
Catalog No.A0234
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the tumor necrosis factor superfamily. The primary function of the encoded transmembrane protein is the induction of apoptosis triggered by binding to FAS. The FAS/FASLG signaling pathway is essential for immune system regulation, including activation-induced cell death (AICD) of T cells and cytotoxic T lymphocyte induced cell death. It has also been implicated in the progression of several cancers. Defects in this gene may be related to some cases of systemic lupus erythematosus (SLE). Alternatively spliced transcript variants have been described.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 100-200 of human FasLG (NP_000630.1).
SequenceGMFQLFHLQKELAELRESTSQMHTASSLEKQIGHPSPPPEKKELRKVAHLTGKSNSRSMPLEWEDTYGIVLLSGVKYKKGGLVINETGLYFVYSKVYFRGQ
Gene ID356
Swiss protP48023
SynonymsFASLG; ALPS1B; APT1LG1; APTL; CD178; CD95-L; CD95L; FASL; TNFSF6; TNLG1A; Fas ligand; FasL; FasLG
Calculated MW14kDa/31kDa
Observed MW46kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilution
  • WB 1:500 - 1:2000
  • IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse heart
Cellular locationCell membrane, Cytoplasmic vesicle lumen, Lysosome lumen, Nucleus, Secreted, Single-pass type II membrane protein
Customer validation

(Jurkat cells)

WB(Homo sapiens, Rattus norvegicus, Mus musculus, Anatinae)

IHC(Rattus norvegicus)

ABclonal:Western blot - FasLG Rabbit pAb (A0234) }

Western blot - FasLG Rabbit pAb (A0234)

Western blot analysis of extracts of Mouse heart, using FasLG antibody (A0234) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 5min.
ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234) }

Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human esophageal cancer using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234) }

Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human tonsil using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234) }

Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human colon using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234) }

Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human placenta using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FasLG Rabbit pAb (A0234) }

Immunohistochemistry - FasLG Rabbit pAb (A0234)

Immunohistochemistry of paraffin-embedded Human lung cancer using FasLG antibody (A0234) at dilution of 1:150 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

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