Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

FMRP Rabbit pAb (A6092)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - FMRP Rabbit pAb (A6092)

Western blot analysis of various lysates, using FMRP antibody (A6092) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - FMRP Rabbit pAb (A6092)

Immunohistochemistry analysis of paraffin-embedded rat lung using FMRP antibody (A6092) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FMRP Rabbit pAb (A6092)

Immunohistochemistry analysis of paraffin-embedded mouse testis using FMRP antibody (A6092) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - FMRP Rabbit pAb (A6092)

Immunohistochemistry analysis of paraffin-embedded mouse brain using FMRP antibody (A6092) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - FMRP Rabbit pAb (A6092)

Immunofluorescence analysis of NIH/3T3 cells using FMRP Rabbit pAb (A6092) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Overview

Product name FMRP Rabbit pAb
Catalog No. A6092
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

The protein encoded by this gene binds RNA and is associated with polysomes. The encoded protein may be involved in mRNA trafficking from the nucleus to the cytoplasm. A trinucleotide repeat (CGG) in the 5' UTR is normally found at 6-53 copies, but an expansion to 55-230 repeats is the cause of fragile X syndrome. Expansion of the trinucleotide repeat may also cause one form of premature ovarian failure (POF1). Multiple alternatively spliced transcript variants that encode different protein isoforms and which are located in different cellular locations have been described for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 500-600 of human FMRP (NP_002015.1).
Sequence SETESDHRDELSDWSLAPTEEERESFLRRGDGRRRGGGGRGQGGRGRGGGFKGNDDHSRTDNRPRNPREAKGRTTDGSLQIRVDCNNERSVHTKTLQNTSS
Gene ID 2332
Swiss prot Q06787
Synonyms POF; FMRP; POF1; FRAXA
Calculated MW 71kDa
Observed MW 60-80kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    
Positive samples 293T, C2C12, Mouse brain, Rat brain
Cellular location Cytoplasm, Nucleus, nucleolus

Documents

Certificate of Compliance

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Lot number

Research Area

FMRP Rabbit pAb images

ABclonal:Western blot - FMRP Rabbit pAb (A6092)}

Western blot - FMRP Rabbit pAb (A6092)

Western blot analysis of various lysates, using FMRP antibody (A6092) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - FMRP Rabbit pAb (A6092)}

Immunohistochemistry - FMRP Rabbit pAb (A6092)

Immunohistochemistry analysis of paraffin-embedded rat lung using FMRP antibody (A6092) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FMRP Rabbit pAb (A6092)}

Immunohistochemistry - FMRP Rabbit pAb (A6092)

Immunohistochemistry analysis of paraffin-embedded mouse testis using FMRP antibody (A6092) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - FMRP Rabbit pAb (A6092)}

Immunohistochemistry - FMRP Rabbit pAb (A6092)

Immunohistochemistry analysis of paraffin-embedded mouse brain using FMRP antibody (A6092) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - FMRP Rabbit pAb (A6092)}

Immunofluorescence - FMRP Rabbit pAb (A6092)

Immunofluorescence analysis of NIH/3T3 cells using FMRP Rabbit pAb (A6092) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on FMR1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to FMR1. (Distance between topics and target gene indicate popularity.) FMR1

* Data provided by citexs.com, for reference only.

Publishing research using A6092? Please let us know so that we can cite the reference in this datasheet.

Antibodies (2)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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