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FBXW7 Rabbit pAb (A5872)

Review (1)Publications (4) Datasheet

ABclonal:Western blot - FBXW7 Polyclonal Antibody (A5872)

Western blot analysis of extracts of various cell lines, using FBXW7 antibody (A5872) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - FBXW7 Polyclonal Antibody (A5872)

Immunohistochemistry of paraffin-embedded mouse lung using FBXW7 antibody (A5872) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - FBXW7 Polyclonal Antibody (A5872)

Immunohistochemistry of paraffin-embedded mouse kidney using FBXW7 antibody (A5872) at dilution of 1:100 (40x lens).

ABclonal:Immunofluorescence - FBXW7 Polyclonal Antibody (A5872)

Immunofluorescence analysis of U2OS cells using FBXW7 antibody (A5872) at dilution of 1:100. Blue: DAPI for nuclear staining.

Review
ABclonal: review for FBXW7 Rabbit pAb(A5872)

Overview

Product nameFBXW7 Rabbit pAb
Catalog No.A5872
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene was previously referred to as FBX30, and belongs to the Fbws class; in addition to an F-box, this protein contains 7 tandem WD40 repeats. This protein binds directly to cyclin E and probably targets cyclin E for ubiquitin-mediated degradation. Mutations in this gene are detected in ovarian and breast cancer cell lines, implicating the gene's potential role in the pathogenesis of human cancers. Multiple transcript variants encoding different isoforms have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 408-707 of human FBXW7 (NP_361014.1).
SequenceAVTGKCLRTLVGHTGGVWSSQMRDNIIISGSTDRTLKVWNAETGECIHTLYGHTSTVRCMHLHEKRVVSGSRDATLRVWDIETGQCLHVLMGHVAAVRCVQYDGRRVVSGAYDFMVKVWDPETETCLHTLQGHTNRVYSLQFDGIHVVSGSLDTSIRVWDVETGNCIHTLTGHQSLTSGMELKDNILVSGNADSTVKIWDIKTGQCLQTLQGPNKHQSAVTCLQFNKNFVITSSDDGTVKLWDLKTGEFIRNLVTLESGGSGGVVWRIRASNTKLVCAVGSRNGTEETKLLVLDFDVDMK
Gene ID55294
Swiss protQ969H0
SynonymsFBXW7; AGO; CDC4; FBW6; FBW7; FBX30; FBXO30; FBXW6; SEL-10; SEL10; hAgo; hCdc4
Calculated MW66kDa/70kDa/79kDa
Observed MW80kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesJurkat, SH-SY5Y, MCF7, mouse testis, rat heart
Cellular locationCytoplasm, Nucleus, nucleolus, nucleoplasm
Customer validation

(BGC823, Homo sapiens)

WB(Mus musculus, Homo sapiens)

IF(Mus musculus)

ABclonal:Western blot - FBXW7 Polyclonal Antibody (A5872) }

Western blot - FBXW7 Polyclonal Antibody (A5872)

Western blot analysis of extracts of various cell lines, using FBXW7 antibody (A5872) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.
ABclonal:Immunohistochemistry - FBXW7 Polyclonal Antibody (A5872) }

Immunohistochemistry - FBXW7 Polyclonal Antibody (A5872)

Immunohistochemistry of paraffin-embedded mouse lung using FBXW7 antibody (A5872) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - FBXW7 Polyclonal Antibody (A5872) }

Immunohistochemistry - FBXW7 Polyclonal Antibody (A5872)

Immunohistochemistry of paraffin-embedded mouse kidney using FBXW7 antibody (A5872) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - FBXW7 Polyclonal Antibody (A5872) }

Immunofluorescence - FBXW7 Polyclonal Antibody (A5872)

Immunofluorescence analysis of U2OS cells using FBXW7 antibody (A5872) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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