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FADD Rabbit mAb (A19049)

Datasheet

ABclonal:Western blot - FADD Rabbit mAb (A19049)

Overview

Product nameFADD Rabbit mAb
Catalog No.A19049
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is an adaptor molecule that interacts with various cell surface receptors and mediates cell apoptotic signals. Through its C-terminal death domain, this protein can be recruited by TNFRSF6/Fas-receptor, tumor necrosis factor receptor, TNFRSF25, and TNFSF10/TRAIL-receptor, and thus it participates in the death signaling initiated by these receptors. Interaction of this protein with the receptors unmasks the N-terminal effector domain of this protein, which allows it to recruit caspase-8, and thereby activate the cysteine protease cascade. Knockout studies in mice also suggest the importance of this protein in early T cell development. [provided by RefSeq, Jul 2008]
ImmunogenA synthesized peptide derived from human FADD
SequenceEmail for sequence
Gene ID8772
Swiss protQ13158
SynonymsGIG3; MORT1; FADD; FADD
Calculated MW28kDa
Observed MW28kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, A-431, U-251MG
Cellular location
ABclonal:Western blot - FADD Rabbit mAb (A19049) }

Western blot - FADD Rabbit mAb (A19049)

Western blot analysis of extracts of various cell lines, using FADD antibody (A19049) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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