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ErbB4/HER4 Rabbit pAb (A0749)

Publication (1) Datasheet

ABclonal:Western blot - ErbB4/HER4 Rabbit pAb (A0749)

Western blot analysis of extracts of rat brain, using ErbB4/HER4/HER4 antibody (A0749) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Overview

Product nameErbB4/HER4 Rabbit pAb
Catalog No.A0749
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1150-1250 of human ErbB4/HER4/HER4 (NP_005226.1).
SequenceYMTPMRDKPKQEYLNPVEENPFVSRRKNGDLQALDNPEYHNASNGPPKAEDEYVNEPLYLNTFANTLGKAEYLKNNILSMPEKAKKAFDNPDYWNHSLPPR
Gene ID2066
Swiss protQ15303
SynonymsALS19; HER4; p180erbB4; ERBB4; HER4/ErbB4
Calculated MW143kDa/145kDa/146kDa
Observed MW147kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesRat brain
Cellular locationCell membrane, Mitochondrion, Nucleus, Single-pass type I membrane protein
Customer validation

WB(Homo sapiens)

IHC(Homo sapiens)

ABclonal:Western blot - ErbB4/HER4 Rabbit pAb (A0749) }

Western blot - ErbB4/HER4 Rabbit pAb (A0749)

Western blot analysis of extracts of rat brain, using ErbB4/HER4/HER4 antibody (A0749) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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