Product Type > Antibodies > Primary Antibodies > Monoclonal Antibodies

EpCAM Mouse mAb (A0895)

Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - EpCAM Mouse mAb (A0895)

Western blot analysis of lysates from HT-29 cells, using EpCAM Mouse mAb (A0895) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name EpCAM Mouse mAb
Catalog No. A0895
Host species Mouse
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Immunogen information

Immunogen Recombinant protein of human EpCAM
Sequence Email for sequence
Gene ID 4072
Swiss prot P16422
Synonyms ESA; KSA; M4S1; MK-1; DIAR5; EGP-2; EGP40; KS1/4; MIC18; TROP1; BerEp4; EGP314; HNPCC8; LYNCH8; MOC-31; Ber-Ep4; TACSTD1; EpCAM
Calculated MW 35kDa
Observed MW 35kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples HT-29
Cellular location Cell junction, Lateral cell membrane, Single-pass type I membrane protein, tight junction

Documents

Certificate of Compliance

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Lot number

Research Area

EpCAM Mouse mAb images

ABclonal:Western blot - EpCAM Mouse mAb (A0895)}

Western blot - EpCAM Mouse mAb (A0895)

Western blot analysis of lysates from HT-29 cells, using EpCAM Mouse mAb (A0895) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on EPCAM. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to EPCAM. (Distance between topics and target gene indicate popularity.) EPCAM

* Data provided by citexs.com, for reference only.

Publishing research using A0895? Please let us know so that we can cite the reference in this datasheet.

Antibodies (6)

Proteins (1)

Secondary Antibodies (17)

* For research use only. Not for therapeutic or diagnostic purposes.

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