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EZH1 Rabbit pAb (A5818)

Publications (2) Datasheet

ABclonal:Western blot - EZH1 Rabbit pAb (A5818)

Western blot analysis of extracts of mouse lung, using EZH1 antibody (A5818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - EZH1 Rabbit pAb (A5818)

Immunofluorescence analysis of A549 cells using EZH1 antibody (A5818). Blue: DAPI for nuclear staining.

Overview

Product nameEZH1 Rabbit pAb
Catalog No.A5818
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
EZH1 is a component of a noncanonical Polycomb repressive complex-2 (PRC2) that mediates methylation of histone H3 (see MIM 602812) lys27 (H3K27) and functions in the maintenance of embryonic stem cell pluripotency and plasticity (Shen et al., 2008 [PubMed 19026780]).[supplied by OMIM, Mar 2009]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 160-280 of human EZH1 (NP_001982.2).
SequenceGEEEMIPGSVLISDAVFLELVDALNQYSDEEEEGHNDTSDGKQDDSKEDLPVTRKRKRHAIEGNKKSSKKQFPNDMIFSAIASMFPENGVPDDMKERYRELTEMSDPNALPPQCTPNIDGP
Gene ID2145
Swiss protQ92800
SynonymsEZH1; KMT6B
Calculated MW68kDa/76kDa/80kDa/85kDa/86kDa
Observed MW85kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesmouse lung
Cellular locationNucleus
Customer validation

IP(Homo sapiens)

WB(Rattus norvegicus)

ABclonal:Western blot - EZH1 Rabbit pAb (A5818) }

Western blot - EZH1 Rabbit pAb (A5818)

Western blot analysis of extracts of mouse lung, using EZH1 antibody (A5818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - EZH1 Rabbit pAb (A5818) }

Immunofluorescence - EZH1 Rabbit pAb (A5818)

Immunofluorescence analysis of A549 cells using EZH1 antibody (A5818). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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