Catalog No:A5818
Host species:Rabbit
Purity:Affinity purification
ABclonal:Western blot - EZH1 antibody (A5818)
ABclonal:Immunofluorescence - EZH1 antibody (A5818)

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Immunogen information

ImmunogenRecombinant funsion protein containing a sequence corresponding to amino acids 160-280 of human EZH1 (NP_001982.2).
Gene ID2145
Swiss ProtQ92800
Calculated MW68kDa/76kDa/80kDa/85kDa/86kDa
Observed MW85kDa


ReactivityHuman, Mouse
Tested applicationsWB, IF
Recommended DilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage BufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesMCF-7, Mouse lung, Mouse brain, Mouse kidney, Mouse spleen
Cellular LocationNucleus
Customer Validation

IP (Human)

EZH1 Polyclonal Antibody images

ABclonal:Western blot - EZH1 antibody (A5818)

Western blot - EZH1 antibody (A5818)

Western blot analysis of extracts of various cell lines, using EZH1 antibody (A5818) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - EZH1 antibody (A5818)

Immunofluorescence - EZH1 antibody (A5818)

Immunofluorescence analysis of A549 cells using EZH1 antibody (A5818). Blue: DAPI for nuclear staining.

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