Products > Catalog Antibodies > Polyclonal Antibodies

ESRα Rabbit pAb (A0296)

Publications (10) Datasheet

Tested applications:WBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIPReactivity:Human, Mouse, Rat

ABclonal:Western blot - ESRα Rabbit pAb (A0296)

Western blot analysis of extracts of HeLa cells, using ESRα antibody (A0296) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product nameESRα Rabbit pAb
Catalog No.A0296
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an estrogen receptor, a ligand-activated transcription factor composed of several domains important for hormone binding, DNA binding, and activation of transcription. The protein localizes to the nucleus where it may form a homodimer or a heterodimer with estrogen receptor 2. Estrogen and its receptors are essential for sexual development and reproductive function, but also play a role in other tissues such as bone. Estrogen receptors are also involved in pathological processes including breast cancer, endometrial cancer, and osteoporosis. Alternative promoter usage and alternative splicing result in dozens of transcript variants, but the full-length nature of many of these variants has not been determined.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human ESRα (ESR1) (NP_000116.2).
SequenceMTMTLHTKASGMALLHQIQGNELEPLNRPQLKIPLERPLGEVYLDSSKPAVYNYPEGAAYEFNAAAAANAQVYGQTGLPYGPGSEAAAFGSNGLGGFPPLNSVSPSPLMLLHPPPQLSPFLQPHGQQVPYYLENEPSGYTVREAGPPAFYRPNSDNRRQGGRERLASTNDKGSMAMESAKETRYCAVCND
Gene ID2099
Swiss protP03372
SynonymsESR1; ER; ESR; ESRA; ESTRR; Era; NR3A1; ER alpha
Calculated MW35kDa/47kDa/53kDa/66kDa
Observed MW66KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:4000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa
Cellular locationCell membrane, Cytoplasm, Cytoplasmic side, Cytoplasmic side, Golgi apparatus, Nucleus, Peripheral membrane protein, Single-pass type I membrane protein
Customer validation

WB(Mus musculus, Homo sapiens)

IHC(Mus musculus)

ABclonal:Western blot - ESRα Rabbit pAb (A0296) }

Western blot - ESRα Rabbit pAb (A0296)

Western blot analysis of extracts of HeLa cells, using ESRα antibody (A0296) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Inquire About This Product

Submit your question about A0296 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A0296? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

Select size and quantity
Contact us to order