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ERMAP Rabbit pAb (A10425)

Datasheet

ABclonal:Western blot - ERMAP Polyclonal Antibody (A10425)

Overview

Product nameERMAP Rabbit pAb
Catalog No.A10425
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a cell surface transmembrane protein that may act as an erythroid cell receptor, possibly as a mediator of cell adhesion. Polymorphisms in this gene are responsible for the Scianna/Radin blood group system. Two transcript variants encoding the same protein have been found for this gene.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 30-155 of human ERMAP (NP_061008.2).
SequenceHAGDAGKFHVALLGGTAELLCPLSLWPGTVPKEVRWLRSPFPQRSQAVHIFRDGKDQDEDLMPEYKGRTVLVRDAQEGSVTLQILDVRLEDQGSYRCLIQVGNLSKEDTVILQVAAPSVGSLSPSA
Gene ID114625
Swiss protQ96PL5
SynonymsERMAP; BTN5; PRO2801; RD; SC
Calculated MW52kDa
Observed MW53kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesBT-474, A-549, K-562, Mouse liver
Cellular locationCell membrane, Cytoplasm, Single-pass type I membrane protein
ABclonal:Western blot - ERMAP Polyclonal Antibody (A10425) }

Western blot - ERMAP Polyclonal Antibody (A10425)

Western blot analysis of extracts of various cell lines, using ERMAP antibody (A10425) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 15s.

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