Products > Catalog Antibodies > Polyclonal Antibodies

ERK1 / ERK2 Rabbit pAb (A16686)

Publications (2) Datasheet

ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)
ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

Overview

Product nameERK1 / ERK2 Rabbit pAb
Catalog No.A16686
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant Protein of human ERK1 / ERK2
SequenceEmail for sequence
Gene ID5594/5595
Swiss protP28482/P27361
SynonymsMAPK1/MAPK3
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42kDa/44kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesHeLa, HepG2, 293T, mouse kidney, mouse brain, rat brain
Cellular location
Customer validation

WB(Homo sapiens, Mus musculus)

IF(Homo sapiens)

    ABclonal:Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Western blot - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (A16686) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 10s.
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunohistochemistry of paraffin-embedded human lung cancer using ERK1 / ERK2 antibody (A16686) at dilution of 1:200 (40x lens).
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunohistochemistry of paraffin-embedded human uterine cancer using ERK1 / ERK2 antibody (A16686) at dilution of 1:200 (40x lens).
    ABclonal:Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunohistochemistry - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunohistochemistry of paraffin-embedded mouse kidney using ERK1 / ERK2 antibody (A16686) at dilution of 1:200 (40x lens).
    ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunofluorescence analysis of C6 cells using ERK1 / ERK2 antibody (A16686) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunofluorescence analysis of NIH/3T3 cells using ERK1 / ERK2 antibody (A16686) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunofluorescence analysis of U2OS cells using ERK1 / ERK2 antibody (A16686) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunofluorescence analysis of C6 cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunofluorescence analysis of NIH-3T3 cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Immunofluorescence analysis of U-2 OS cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686) }

    Immunofluorescence - ERK1 / ERK2 Polyclonal Antibody (A16686)

    Confocal immunofluorescence analysis of Hela cells using ERK1 / ERK2 Polyclonal Antibody (A16686) at dilution of 1:200. Blue: DAPI for nuclear staining.

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