Products > Catalog Antibodies > Monoclonal Antibodies

ERK1 / ERK2 Mouse mAb (A10613)

Review (1)Publications (12) Datasheet

ABclonal:Western blot - ERK1 / ERK2 Mouse mAb (A10613)

Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (A10613) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 180s.

Review
ABclonal: review for ERK1 / ERK2 Mouse mAb(A10613)

Overview

Product nameERK1 / ERK2 Mouse mAb
Catalog No.A10613
Host speciesMouse
Purification methodAffinity purification
IsotypeIgG
MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets.
ImmunogenA synthetic peptide of human ERK1 / ERK2
SequenceEmail for sequence
Gene ID5594/5595
Swiss protP28482/P27361
Synonyms
Calculated MW36kDa/41kDa/38kDa/40kDa/43kDa
Observed MW42KDa/44KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:1000 - 1:2000
IHC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    
Positive samplesMouse brain, Rat brain
Cellular location
Customer validation

(mouse cells)

WB(Rattus norvegicus, Mus musculus, Homo sapiens)

IHC(Mus musculus)

IF(Mus musculus)

    ABclonal:Western blot - ERK1 / ERK2 Mouse mAb (A10613) }

    Western blot - ERK1 / ERK2 Mouse mAb (A10613)

    Western blot analysis of extracts of various cell lines, using ERK1 / ERK2 antibody (A10613) at 1:1000 dilution.
    Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
    Lysates/proteins: 25ug per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Submit an review for A10613

    Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

    Reviews List

    Inquire About This Product

    Submit your question about A10613 below and we will get back to you within one business day.

    Alternatively, call us at 888.754.5670.

    Publishing research using A10613? Please let us know so that we can cite the reference in this datasheet.

    Select size and quantity
    Contact us to order