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ERCC1 Polyclonal Antibody (A5291)

Datasheet

ABclonal:Western blot - ERCC1 antibody (A5291)
ABclonal:Immunofluorescence - ERCC1 antibody (A5291)
ABclonal:Immunofluorescence - ERCC1 antibody (A5291)

Overview

Product nameERCC1 Polyclonal Antibody
Catalog No.A5291
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The product of this gene functions in the nucleotide excision repair pathway, and is required for the repair of DNA lesions such as those induced by UV light or formed by electrophilic compounds including cisplatin. The encoded protein forms a heterodimer with the XPF endonuclease (also known as ERCC4), and the heterodimeric endonuclease catalyzes the 5' incision in the process of excising the DNA lesion. The heterodimeric endonuclease is also involved in recombinational DNA repair and in the repair of inter-strand crosslinks. Mutations in this gene result in cerebrooculofacioskeletal syndrome, and polymorphisms that alter expression of this gene may play a role in carcinogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. The last exon of this gene overlaps with the CD3e molecule, epsilon associated protein gene on the opposite strand.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human ERCC1 (NP_973730.1).
SequenceMDPGKDKEGVPQPSGPPARKKFVIPLDEDEVPPGVAKPLFRSTQSLPTVDTSAQAAPQTYAEYAISQPLEGAGATCPTGSEPLAGETPNQALKPGAKSNSIIVSPRQRGNPVLKFVRNVPWEFGDVIPDYVLGQSTCALFLSLRYHNLHPDYIHGRLQSLGKNFALRVLLVQVDVKDPQQALKELAKMCILADCTLILAWSPEEAGRYLETYKAYEQKPADLLMEKLEQDFVSRVTECLTTVKSVNKTDSQTLLTTFGSLEQLIAASREDLALCPGLGPQKVRALGKNPRSWGKERAPNKHNLRPQSFKVKKEPKTRHSGFRL
Gene ID2067
Swiss protP07992
SynonymsCOFS4; RAD10; UV20
Calculated MW25kDa/29kDa/32kDa/35kDa
Observed MW38kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunofluorescence    
Positive samplesRaji, MCF-7, PC-3, HepG2, HeLa, Mouse liver, Mouse brain
Cellular locationCytoplasm, Nucleus
ABclonal:Western blot - ERCC1 antibody (A5291)}

Western blot - ERCC1 antibody (A5291)

Western blot analysis of extracts of various cell lines, using ERCC1 antibody (A5291) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunofluorescence - ERCC1 antibody (A5291)}

Immunofluorescence - ERCC1 antibody (A5291)

Immunofluorescence analysis of U2OS cells using ERCC1 antibody (A5291). Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - ERCC1 antibody (A5291)}

Immunofluorescence - ERCC1 antibody (A5291)

Immunofluorescence analysis of HeLa cells using ERCC1 antibody (A5291).

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