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eIF2α Rabbit pAb (A0764)

Review (1)Publications (5) Datasheet

ABclonal:Western blot - eIF2α Rabbit pAb (A0764)
ABclonal:Immunohistochemistry - eIF2α Rabbit pAb (A0764)
ABclonal:Immunohistochemistry - eIF2α Rabbit pAb (A0764)
ABclonal:Immunohistochemistry - eIF2α Rabbit pAb (A0764)
ABclonal:Immunofluorescence - eIF2α Rabbit pAb (A0764)
Review
ABclonal: review for eIF2α Rabbit pAb(A0764)

Overview

Product nameeIF2α Rabbit pAb
Catalog No.A0764
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The translation initiation factor EIF2 catalyzes the first regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, EIF2, and GTP. EIF2 is composed of 3 nonidentical subunits, the 36-kD EIF2-alpha subunit (EIF2S1), the 38-kD EIF2-beta subunit (EIF2S2; MIM 603908), and the 52-kD EIF2-gamma subunit (EIF2S3; MIM 300161). The rate of formation of the ternary complex is modulated by the phosphorylation state of EIF2-alpha (Ernst et al., 1987 [PubMed 2948954]).[supplied by OMIM, Feb 2010]
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human eIF2α (NP_004085.1).
SequenceMPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKE
Gene ID1965
Swiss protP05198
SynonymsEIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; EIF2S1
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesLO2, Jurkat, MCF7, SW480, SKOV3, Mouse kidney, Mouse spleen
Cellular locationCytoplasmic granule
Customer validation

(293T cells)

WB(Mus musculus, Rattus norvegicus, Homo sapiens)

ABclonal:Western blot - eIF2α Rabbit pAb (A0764) }

Western blot - eIF2α Rabbit pAb (A0764)

Western blot analysis of extracts of various cell lines, using eIF2α antibody (A0764) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - eIF2α Rabbit pAb (A0764) }

Immunohistochemistry - eIF2α Rabbit pAb (A0764)

Immunohistochemistry of paraffin-embedded rat heart using eIF2α antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - eIF2α Rabbit pAb (A0764) }

Immunohistochemistry - eIF2α Rabbit pAb (A0764)

Immunohistochemistry of paraffin-embedded human liver cancer using eIF2α antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - eIF2α Rabbit pAb (A0764) }

Immunohistochemistry - eIF2α Rabbit pAb (A0764)

Immunohistochemistry of paraffin-embedded mouse heart using eIF2α antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - eIF2α Rabbit pAb (A0764) }

Immunofluorescence - eIF2α Rabbit pAb (A0764)

Immunofluorescence analysis of PC-12 cells using eIF2α antibody (A0764) at dilution of 1:100. Blue: DAPI for nuclear staining.

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