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EIF2S1 Polyclonal Antibody (A0764)

Review (1)Publication (2) Datasheet

ABclonal:Western blot - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)
ABclonal:Immunofluorescence - EIF2S1 Polyclonal Antibody (A0764)
Review
ABclonal: review for EIF2S1 Polyclonal Antibody(A0764)

Overview

Product nameEIF2S1 Polyclonal Antibody
Catalog No.A0764
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human EIF2S1 (NP_004085.1).
SequenceMPGLSCRFYQHKFPEVEDVVMVNVRSIAEMGAYVSLLEYNNIEGMILLSELSRRRIRSINKLIRIGRNECVVVIRVDKEKGYIDLSKRRVSPEEAIKCEDKFTKSKTVYSILRHVAEVLEYTKDEQLESLFQRTAWVFDDKYKRPGYGAYDAFKHAVSDPSILDSLDLNEDEREVLINNINRRLTPQAVKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKE
Gene ID1965
Swiss protP05198
SynonymsEIF2S1; EIF-2; EIF-2A; EIF-2alpha; EIF2; EIF2A; eukaryotic translation initiation factor 2 subunit 1
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesLO2, Jurkat, MCF7, SW480, SKOV3, Mouse kidney, Mouse spleen
Cellular locationCytoplasmic granule
Customer validation

WB(Mouse, Human)

ABclonal:Western blot - EIF2S1 Polyclonal Antibody (A0764) }

Western blot - EIF2S1 Polyclonal Antibody (A0764)

Western blot analysis of extracts of various cell lines, using EIF2S1 antibody (A0764) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764) }

Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)

Immunohistochemistry of paraffin-embedded rat heart using EIF2S1 antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764) }

Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)

Immunohistochemistry of paraffin-embedded human liver cancer using EIF2S1 antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764) }

Immunohistochemistry - EIF2S1 Polyclonal Antibody (A0764)

Immunohistochemistry of paraffin-embedded mouse heart using EIF2S1 antibody (A0764) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - EIF2S1 Polyclonal Antibody (A0764) }

Immunofluorescence - EIF2S1 Polyclonal Antibody (A0764)

Immunofluorescence analysis of PC-12 cells using EIF2S1 antibody (A0764) at dilution of 1:100. Blue: DAPI for nuclear staining.

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