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EGFR Polyclonal Antibody (A2909)

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ABclonal:Western blot - EGFR Polyclonal Antibody (A2909)
Review
ABclonal: review for EGFR Polyclonal Antibody(A2909)
Review
ABclonal: review for EGFR Polyclonal Antibody(A2909)
Review
ABclonal: review for EGFR Polyclonal Antibody(A2909)

Overview

Product nameEGFR Polyclonal Antibody
Catalog No.A2909
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene is a transmembrane glycoprotein that is a member of the protein kinase superfamily. This protein is a receptor for members of the epidermal growth factor family. EGFR is a cell surface protein that binds to epidermal growth factor. Binding of the protein to a ligand induces receptor dimerization and tyrosine autophosphorylation and leads to cell proliferation. Mutations in this gene are associated with lung cancer.
ImmunogenA synthetic peptide of human EGFR
SequenceEmail for sequence
Gene ID1956
Swiss protP00533
SynonymsEGFR; ERBB; ERBB1; HER1; NISBD2; PIG61; mENA
Calculated MW44kDa/69kDa/77kDa/134kDa
Observed MW180kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, HepG2, OVCAR3
Cellular locationCell membrane, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus membrane, Nucleus membrane, Nucleus, Secreted, Single-pass type I membrane protein
Customer validation

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WB(Human, Mouse)

ABclonal:Western blot - EGFR Polyclonal Antibody (A2909) }

Western blot - EGFR Polyclonal Antibody (A2909)

Western blot analysis of extracts of various cell lines, using EGFR antibody (A2909) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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