Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

EG5/KIF11 Rabbit pAb (A7907)

Publications (2) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human

ABclonal:Western blot - EG5/KIF11 Rabbit pAb (A7907)

Western blot analysis of various lysates using EG5/KIF11 Rabbit pAb (A7907) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunofluorescence - EG5/KIF11 Rabbit pAb (A7907)

Immunofluorescence analysis of U2OS cells using EG5/KIF11 Rabbit pAb (A7907) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Overview

Product name EG5/KIF11 Rabbit pAb
Catalog No. A7907
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes a motor protein that belongs to the kinesin-like protein family. Members of this protein family are known to be involved in various kinds of spindle dynamics. The function of this gene product includes chromosome positioning, centrosome separation and establishing a bipolar spindle during cell mitosis.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 797-1056 of human EG5/EG5/KIF11 (NP_004514.2).
Sequence SVKHSDKLNGNLEKISQETEQRCESLNTRTVYFSEQWVSSLNEREQELHNLLEVVSQCCEASSSDITEKSDGRKAAHEKQHNIFLDQMTIDEDKLIAQNLELNETIKIGLTKLNCFLEQDLKLDIPTGTTPQRKSYLYPSTLVRTEPREHLLDQLKRKQPELLMMLNCSENNKEETIPDVDVEEAVLGQYTEEPLSQEPSVDAGVDCSSIGGVPFFQHKKSHGKDKENRGINTLERSKVEETTEHLVTKSRLPLRAQINL
Gene ID 3832
Swiss prot P52732
Synonyms EG5; HKSP; KNSL1; MCLMR; TRIP5; EG5/KIF11
Calculated MW 119kDa
Observed MW 130kDa

Applications

Reactivity Human
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IF/ICC 1:50 - 1:100
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application key Western blotting    Immunofluorescence    
Positive samples HeLa, Jurkat, SW620
Cellular location Cytoplasm, cytoskeleton, spindle pole
Customer validation

WB (Homo sapiens)

IF (Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

EG5/KIF11 Rabbit pAb images

ABclonal:Western blot - EG5/KIF11 Rabbit pAb (A7907)}

Western blot - EG5/KIF11 Rabbit pAb (A7907)

Western blot analysis of various lysates using EG5/KIF11 Rabbit pAb (A7907) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.
ABclonal:Immunofluorescence - EG5/KIF11 Rabbit pAb (A7907)}

Immunofluorescence - EG5/KIF11 Rabbit pAb (A7907)

Immunofluorescence analysis of U2OS cells using EG5/KIF11 Rabbit pAb (A7907) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

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Related Targets

Based on existing publications, the following genes are closely related to research on KIF11. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to KIF11. (Distance between topics and target gene indicate popularity.) KIF11

* Data provided by citexs.com, for reference only.

Publishing research using A7907? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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