Products > Catalog Antibodies > Polyclonal Antibodies

E-Cadherin Rabbit pAb (A11492)

Review (1)Publications (6) Datasheet

ABclonal:Western blot - E-Cadherin Rabbit pAb (A11492)

Western blot analysis of extracts of various cell lines, using E-Cadherin antibody (A11492) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A11492)

Immunohistochemistry of paraffin-embedded Rat kidney using E-Cadherin antibody (A11492) at dilution of 1:50 (40x lens).

ABclonal:Immunofluorescence - E-Cadherin Rabbit pAb (A11492)

Immunofluorescence analysis of A431 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Review
ABclonal: review for E-Cadherin Rabbit pAb(A11492)

Overview

Product nameE-Cadherin Rabbit pAb
Catalog No.A11492
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a classical cadherin of the cadherin superfamily. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature glycoprotein. This calcium-dependent cell-cell adhesion protein is comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Mutations in this gene are correlated with gastric, breast, colorectal, thyroid and ovarian cancer. Loss of function of this gene is thought to contribute to cancer progression by increasing proliferation, invasion, and/or metastasis. The ectodomain of this protein mediates bacterial adhesion to mammalian cells and the cytoplasmic domain is required for internalization. This gene is present in a gene cluster with other members of the cadherin family on chromosome 16.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 700-800 of human E-Cadherin (NP_004351.1).
SequencePVEAGLQIPAILGILGGILALLILILLLLLFLRRRAVVKEPLLPPEDDTRDNVYYYDEEGGGEEDQDFDLSQLHRGLDARPEVTRNDVAPTLMSVPRYLPR
Gene ID999
Swiss protP12830
SynonymsArc-1; CD324; CDHE; ECAD; LCAM; UVO; CDH1; E-Cadherin
Calculated MW90kDa/97kDa
Observed MW130KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:1000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesMCF7, Rat liver
Cellular locationCell junction, Cell membrane, Endosome, Golgi apparatus, Single-pass type I membrane protein, trans-Golgi network
Customer validation

(Human lung cancer cell)

WB(Homo sapiens, Mus musculus)

IHC(Homo sapiens)

ABclonal:Western blot - E-Cadherin Rabbit pAb (A11492) }

Western blot - E-Cadherin Rabbit pAb (A11492)

Western blot analysis of extracts of various cell lines, using E-Cadherin antibody (A11492) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - E-Cadherin Rabbit pAb (A11492) }

Immunohistochemistry - E-Cadherin Rabbit pAb (A11492)

Immunohistochemistry of paraffin-embedded Rat kidney using E-Cadherin antibody (A11492) at dilution of 1:50 (40x lens).
ABclonal:Immunofluorescence - E-Cadherin Rabbit pAb (A11492) }

Immunofluorescence - E-Cadherin Rabbit pAb (A11492)

Immunofluorescence analysis of A431 cells using E-Cadherin Rabbit pAb (A11492) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11492

Please submit reviews to your technical sales specialist directly. Alternatively, email us at service@abclonal.com

Reviews List

Inquire About This Product

Submit your question about A11492 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A11492? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μL sample size available Add to Cart
Contact us to order