Products > Catalog Antibodies > Methylated Antibodies

DiMethyl-Histone H3-K9 pAb (A2359)

Reviews (2)Publications (4) Datasheet

ABclonal:Western blot - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Dot Blot - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K9 pAb (A2359)
ABclonal:Chromatin Immunoprecipitation - DiMethyl-Histone H3-K9 pAb (A2359)
Review
ABclonal:Chromatin Immunoprecipitation (ChIP) review for DiMethyl-Histone H3-K9 pAb(A2359)
Review
ABclonal:Western blot (WB) review for DiMethyl-Histone H3-K9 pAb(A2359)

Overview

Product nameDiMethyl-Histone H3-K9 pAb
Catalog No.A2359
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K9 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsHIST3H3; H3.4; H3/g; H3FT; H3t; histone H3.1t
Calculated MW15kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:50 - 1:200
CHIPseq 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, H3 protein
Cellular locationChromosome, Nucleus
Customer validation

ChIP(UBQ10)

WB(293FT,PC9, Human, Mouse)

ABclonal:Western blot - DiMethyl-Histone H3-K9 pAb (A2359) }

Western blot - DiMethyl-Histone H3-K9 pAb (A2359)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K9 antibody (A2359).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal:Dot Blot - DiMethyl-Histone H3-K9 pAb (A2359) }

Dot Blot - DiMethyl-Histone H3-K9 pAb (A2359)

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K9 antibody (A2359).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)

Immunohistochemistry of paraffin-embedded rat liver using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)

Immunohistochemistry of paraffin-embedded human thyroid cancer using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)

Immunohistochemistry of paraffin-embedded human rectal cancer using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)

Immunohistochemistry of paraffin-embedded rat lung using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)

Immunohistochemistry of paraffin-embedded rat testis using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)

Immunohistochemistry of paraffin-embedded rat brain using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunohistochemistry - DiMethyl-Histone H3-K9 pAb (A2359)

Immunohistochemistry of paraffin-embedded mouse kidney using DiMethyl-Histone H3-K9 antibody (A2359) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K9 pAb (A2359) }

Immunofluorescence - DiMethyl-Histone H3-K9 pAb (A2359)

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K9 antibody (A2359). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - DiMethyl-Histone H3-K9 pAb (A2359) }

Chromatin Immunoprecipitation - DiMethyl-Histone H3-K9 pAb (A2359)

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3-K9 antibody (A2359) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Submit an review for A2359

  1. Please choose your application, enter the information requested below. All fields are mandatory, except the ones marked as optional.
  2. When you're happy with the review, submit it by clicking on the Submit button.
1:  
H(s)     M(s)
H(s)      M(s)     Second(s)
(allowed file types: .jpg, .png, .tif, .bmp, .gif)
kDa

Reviews List

Inquire About This Product

Submit your question about A2359 below and we will get back to you within one business day.

Alternatively, call us at 888.754.5670.

Publishing research using A2359? Please let us know so that we can cite the reference in this datasheet.

Select size and quantity
5 μl sample size available Add to Cart
Add to Cart

or Request quote for bulk purchase

Available for next day delivery (US only)

Contact us to order