Products > Catalog Antibodies > Methylated Antibodies

DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Reviews (3) Datasheet

ABclonal:Western blot - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)
ABclonal: - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)
ABclonal:Chromatin Immunoprecipitation - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)
Review
ABclonal: review for DiMethyl-Histone H3-K27 Rabbit pAb(A2362)
Review
ABclonal: review for DiMethyl-Histone H3-K27 Rabbit pAb(A2362)
Review
ABclonal: review for DiMethyl-Histone H3-K27 Rabbit pAb(A2362)

Overview

Product nameDiMethyl-Histone H3-K27 Rabbit pAb
Catalog No.A2362
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene is intronless and encodes a replication-dependent histone that is a member of the histone H3 family. Transcripts from this gene lack polyA tails; instead, they contain a palindromic termination element. This gene is located separately from the other H3 genes that are in the histone gene cluster on chromosome 6p22-p21.3.
ImmunogenA synthetic methylated peptide corresponding to residues surrounding K27 of human histone H3
SequenceEmail for sequence
Gene ID8290
Swiss protQ16695
SynonymsH3.4; H3/g; H3FT; H3t; HIST3H3; Histone H3; HIST1H3A
Calculated MW15kDa
Observed MW18kDa
ReactivityHuman, Mouse, Rat, Other (Wide Range)
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide ArrayDB
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:200
ChIP 1:20 - 1:100
ChIPseq 1:20 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa
Cellular locationChromosome, Nucleus
Customer validation

(Goat sperm, HeLa, 293FT,PC9)

ABclonal:Western blot - DiMethyl-Histone H3-K27 Rabbit pAb (A2362) }

Western blot - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Western blot analysis of extracts of various cell lines, using DiMethyl-Histone H3-K27 antibody (A2362).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
ABclonal: - DiMethyl-Histone H3-K27 Rabbit pAb (A2362) }

- DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Dot-blot analysis of all sorts of methylation peptides using DiMethyl-Histone H3-K27 antibody (A2362) at 1:1000 dilution.
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362) }

Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Immunohistochemistry of paraffin-embedded human kidney cancer using DiMethyl-Histone H3-K27 antibody (A2362) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362) }

Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Immunohistochemistry of paraffin-embedded Mouse heart using DiMethyl-Histone H3-K27 antibody (A2362) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362) }

Immunohistochemistry - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Immunohistochemistry of paraffin-embedded Rat brain using DiMethyl-Histone H3-K27 antibody (A2362) at dilution of 1:100 (40x lens).
ABclonal:Immunofluorescence - DiMethyl-Histone H3-K27 Rabbit pAb (A2362) }

Immunofluorescence - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Immunofluorescence analysis of 293T cells using DiMethyl-Histone H3-K27 antibody (A2362). Blue: DAPI for nuclear staining.
ABclonal:Chromatin Immunoprecipitation - DiMethyl-Histone H3-K27 Rabbit pAb (A2362) }

Chromatin Immunoprecipitation - DiMethyl-Histone H3-K27 Rabbit pAb (A2362)

Chromatin immunoprecipitation analysis of extracts of 293 cell line, using DiMethyl-Histone H3-K27 antibody (A2362) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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