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DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

Datasheet

ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product nameDiMethyl-DNMT3A-K44 Rabbit pAb
Catalog No.A16012
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated.
ImmunogenA synthetic dimethylated peptide around K44 of mouse DNMT3A (NP_031898.1).
SequenceARKVG
Gene ID13435
Swiss protO88508
SynonymsDnmt3a; MmuIIIA; DNMT3A2; M.HsaIIIA; TBRS
Calculated MW77kDa/101kDa
Observed MW
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionIF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyImmunofluorescence    
Positive samples
Cellular locationCytoplasm, Nucleus
    ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012) }

    Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

    Immunofluorescence analysis of U2OS cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012) }

    Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

    Immunofluorescence analysis of C6 cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012) }

    Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

    Immunofluorescence analysis of HeLa cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.
    ABclonal:Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012) }

    Immunofluorescence - DiMethyl-DNMT3A-K44 Rabbit pAb (A16012)

    Immunofluorescence analysis of NIH/3T3 cells using DiMethyl-DNMT3A-K44 antibody (A16012) at dilution of 1:100. Blue: DAPI for nuclear staining.

    * For research use only. Not for therapeutic or diagnostic purposes.

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