Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

DRD2 Rabbit pAb (A12930)

Publications (5) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - DRD2 Rabbit pAb (A12930)

Western blot analysis of U-251MG, using DRD2 antibody (A12930) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Overview

Product name DRD2 Rabbit pAb
Catalog No. A12930
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes the D2 subtype of the dopamine receptor. This G-protein coupled receptor inhibits adenylyl cyclase activity. A missense mutation in this gene causes myoclonus dystonia; other mutations have been associated with schizophrenia. Alternative splicing of this gene results in two transcript variants encoding different isoforms. A third variant has been described, but it has not been determined whether this form is normal or due to aberrant splicing.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DRD2 (NP_000786.1).
Sequence LERQNWSRPFNGSDGK
Gene ID 1813
Swiss prot P14416
Synonyms D2R; D2DR; DRD2
Calculated MW 51kDa
Observed MW 51kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples U-251MG
Cellular location Cell membrane, Multi-pass membrane protein
Customer validation

WB (Mus musculus, Rattus norvegicus, Homo sapiens)

IHC (Danio rerio)

Documents

Certificate of Compliance

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Lot number

Research Area

DRD2 Rabbit pAb images

ABclonal:Western blot - DRD2 Rabbit pAb (A12930)}

Western blot - DRD2 Rabbit pAb (A12930)

Western blot analysis of U-251MG, using DRD2 antibody (A12930) at 1:400 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on DRD2. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DRD2. (Distance between topics and target gene indicate popularity.) DRD2

* Data provided by citexs.com, for reference only.

Publishing research using A12930? Please let us know so that we can cite the reference in this datasheet.

* For research use only. Not for therapeutic or diagnostic purposes.

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