Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

DNMT1 Rabbit pAb (A17474)

Datasheet COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - DNMT1 Rabbit pAb (A17474)

Western blot analysis of lysates from Jurkat cells, using DNMT1 Rabbit pAb (A17474) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A17474)

Immunohistochemistry analysis of DNMT1 in paraffin-embedded human tonsil using DNMT1 Rabbit pAb (A17474) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A17474)

Immunohistochemistry analysis of DNMT1 in paraffin-embedded mouse spleen using DNMT1 Rabbit pAb (A17474) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A17474)

Immunofluorescence analysis of U-2 OS cells using DNMT1 Rabbit pAb (A17474) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - DNMT1 Rabbit pAb (A17474)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg DNMT1 antibody (A17474). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A17474) at a dilution of 1:1000.

ABclonal:Chromatin Immunoprecipitation - DNMT1 Rabbit pAb (A17474)

Chromatin immunoprecipitation analysis of extracts of Jurkat cells, using DNMT1 antibody (A17474) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Overview

Product name DNMT1 Rabbit pAb
Catalog No. A17474
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.

Immunogen information

Immunogen Recombinant fusion protein containing a sequence corresponding to amino acids 760-1109 of DNMT1 (NP_001370.1).
Sequence GDCVSVIPDDSSKPLYLARVTALWEDSSNGQMFHAHWFCAGTDTVLGATSDPLELFLVDECEDMQLSYIHSKVKVIYKAPSENWAMEGGMDPESLLEGDDGKTYFYQLWYDQDYARFESPPKTQPTEDNKFKFCVSCARLAEMRQKEIPRVLEQLEDLDSRVLYYSATKNGILYRVGDGVYLPPEAFTFNIKLSSPVKRPRKEPVDEDLYPEHYRKYSDYIKGSNLDAPEPYRIGRIKEIFCPKKSNGRPNETDIKIRVNKFYRPENTHKSTPASYHADINLLYWSDEEAVVDFKAVQGRCTVEYGEDLPECVQVYSMGGPNRFYFLEAYNAKSKSFEDPPNHARSPGNK
Gene ID 1786
Swiss prot P26358
Synonyms AIM; DNMT; MCMT; CXXC9; HSN1E; ADCADN; m.HsaI; DNMT1
Calculated MW 183kDa
Observed MW 200kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
  • ChIP 3μg antibody for 10μg-15μg of Chromatin
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Application key Western blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples Jurkat
Cellular location Nucleus

Documents

Certificate of Compliance

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Lot number

Research Area

DNMT1 Rabbit pAb images

ABclonal:Western blot - DNMT1 Rabbit pAb (A17474)}

Western blot - DNMT1 Rabbit pAb (A17474)

Western blot analysis of lysates from Jurkat cells, using DNMT1 Rabbit pAb (A17474) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.
ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A17474)}

Immunohistochemistry - DNMT1 Rabbit pAb (A17474)

Immunohistochemistry analysis of DNMT1 in paraffin-embedded human tonsil using DNMT1 Rabbit pAb (A17474) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A17474)}

Immunohistochemistry - DNMT1 Rabbit pAb (A17474)

Immunohistochemistry analysis of DNMT1 in paraffin-embedded mouse spleen using DNMT1 Rabbit pAb (A17474) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A17474)}

Immunofluorescence - DNMT1 Rabbit pAb (A17474)

Immunofluorescence analysis of U-2 OS cells using DNMT1 Rabbit pAb (A17474) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - DNMT1 Rabbit pAb (A17474)}

Immunoprecipitation - DNMT1 Rabbit pAb (A17474)

Immunoprecipitation analysis of 300 μg extracts of Jurkat cells using 3 μg DNMT1 antibody (A17474). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A17474) at a dilution of 1:1000.
ABclonal:Chromatin Immunoprecipitation - DNMT1 Rabbit pAb (A17474)}

Chromatin Immunoprecipitation - DNMT1 Rabbit pAb (A17474)

Chromatin immunoprecipitation analysis of extracts of Jurkat cells, using DNMT1 antibody (A17474) and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

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Related Targets

Based on existing publications, the following genes are closely related to research on DNMT1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DNMT1. (Distance between topics and target gene indicate popularity.) DNMT1

* Data provided by citexs.com, for reference only.

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* For research use only. Not for therapeutic or diagnostic purposes.

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