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DNMT1 Polyclonal Antibody (A5495)

Reviews (10)Publications (3) Datasheet

ABclonal:Western blot - DNMT1 Polyclonal Antibody (A5495)
ABclonal:Immunofluorescence - DNMT1 Polyclonal Antibody (A5495)

Overview

Product nameDNMT1 Polyclonal Antibody
Catalog No.A5495
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human DNMT1 (NP_001370.1).
SequenceMPARTAPARVPTLAVPAISLPDDVRRRLKDLERDSLTEKECVKEKLNLLHEFLQTEIKNQLCDLETKLRKEELSEEGYLAKVKSLLNKDLSLENGAHAYNREVNGRLENGNQARSEARRVGMADANSPPKPLSKPRTPRRSKSDGEAKPEPSPSPRITRKSTRQTTITSHFAKGPAKRKPQEESERAKSDESIKEEDKDQDEKRRRVTSRERVARPLPAEEPERAKSGTRTEKEEERDEKEEKRLRSQTKEPTPKQKLKEEPDREARAGV
Gene ID1786
Swiss protP26358
SynonymsADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
Calculated MW144kDa/183kDa/184kDa
Observed MW200kDa
ReactivityHuman, Rat
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFC
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samples293T, SW480
Cellular locationNucleus
Customer validation

WB(Human hepatocellular carcinoma cell line, HUVEC, SMMC7721,BEL7404, Human cells, HEK293T, BEL-7402)

ChIP(Human)

IF(Human)

ABclonal:Western blot - DNMT1 Polyclonal Antibody (A5495) }

Western blot - DNMT1 Polyclonal Antibody (A5495)

Western blot analysis of extracts of various cell lines, using DNMT1 antibody (A5495) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 10s.
ABclonal:Immunofluorescence - DNMT1 Polyclonal Antibody (A5495) }

Immunofluorescence - DNMT1 Polyclonal Antibody (A5495)

Immunofluorescence analysis of 293T cells using DNMT1 antibody (A5495) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

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