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DNMT1 Rabbit pAb (A16729)

Publications (2) Datasheet

ABclonal:Western blot - DNMT1 Rabbit pAb (A16729)

Western blot analysis of extracts of HeLa cells, using DNMT1 antibody (A16729) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Western blot - DNMT1 Rabbit pAb (A16729)

Western blot analysis of extracts of various cell lines, using DNMT1 antibody (A16729) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Western blot - DNMT1 Rabbit pAb (A16729)

Western blot analysis of extracts of 293T cells, using DNMT1 antibody (A16729) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A16729)

Immunohistochemistry of paraffin-embedded human breast cancer using DNMT1 antibody (A16729) at dilution of 1:200 (40x lens).

ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A16729)

Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (A16729) at dilution of 1:200 (40x lens).

ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Immunofluorescence analysis of C6 cells using DNMT1 antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Immunofluorescence analysis of NIH/3T3 cells using DNMT1 antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal Antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal Antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - DNMT1 Rabbit pAb (A16729)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug DNMT1 antibody (A16729). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A16729) at a dilition of 1:1000.

Overview

Product nameDNMT1 Rabbit pAb
Catalog No.A16729
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants.
ImmunogenRecombinant protein of human DNMT1.
SequenceEmail for sequence
Gene ID1786
Swiss protP26358
SynonymsADCADN; AIM; CXXC9; DNMT; HSN1E; MCMT; m.HsaI; DNMT1
Calculated MW144kDa/183kDa/184kDa
Observed MW200KDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IF 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samples293T, HeLa, Mouse brain, Rat brain
Cellular locationNucleus
Customer validation

WB(Rattus norvegicus, Homo sapiens, Mus musculus)

IF(Homo sapiens,Mus musculus)

ABclonal:Western blot - DNMT1 Rabbit pAb (A16729) }

Western blot - DNMT1 Rabbit pAb (A16729)

Western blot analysis of extracts of HeLa cells, using DNMT1 antibody (A16729) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Western blot - DNMT1 Rabbit pAb (A16729) }

Western blot - DNMT1 Rabbit pAb (A16729)

Western blot analysis of extracts of various cell lines, using DNMT1 antibody (A16729) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.
ABclonal:Western blot - DNMT1 Rabbit pAb (A16729) }

Western blot - DNMT1 Rabbit pAb (A16729)

Western blot analysis of extracts of 293T cells, using DNMT1 antibody (A16729) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.
ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A16729) }

Immunohistochemistry - DNMT1 Rabbit pAb (A16729)

Immunohistochemistry of paraffin-embedded human breast cancer using DNMT1 antibody (A16729) at dilution of 1:200 (40x lens).
ABclonal:Immunohistochemistry - DNMT1 Rabbit pAb (A16729) }

Immunohistochemistry - DNMT1 Rabbit pAb (A16729)

Immunohistochemistry of paraffin-embedded human tonsil using DNMT1 antibody (A16729) at dilution of 1:200 (40x lens).
ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729) }

Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Immunofluorescence analysis of C6 cells using DNMT1 antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729) }

Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Immunofluorescence analysis of NIH/3T3 cells using DNMT1 antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729) }

Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal Antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - DNMT1 Rabbit pAb (A16729) }

Immunofluorescence - DNMT1 Rabbit pAb (A16729)

Confocal immunofluorescence analysis of HeLa cells using DNMT1 Polyclonal Antibody (A16729) at dilution of 1:100. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - DNMT1 Rabbit pAb (A16729) }

Immunoprecipitation - DNMT1 Rabbit pAb (A16729)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug DNMT1 antibody (A16729). Western blot was performed from the immunoprecipitate using DNMT1 antibody (A16729) at a dilition of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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