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DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Publication (1) Datasheet

ABclonal:Western blot - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Western blot analysis of extracts of various cell lines, using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Immunohistochemistry of paraffin-embedded human breast using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at dilution of 1:100 (40x lens).

ABclonal:Immunohistochemistry - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Immunohistochemistry of paraffin-embedded human gastric cancer using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at dilution of 1:100 (40x lens).

ABclonal:Immunoprecipitation - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741). Western blot was performed from the immunoprecipitate using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at a dilition of 1:500.

Overview

Product nameDNA topoisomerase I (TOP1) Rabbit pAb
Catalog No.A7741
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This enzyme catalyzes the transient breaking and rejoining of a single strand of DNA which allows the strands to pass through one another, thus altering the topology of DNA. This gene is localized to chromosome 20 and has pseudogenes which reside on chromosomes 1 and 22.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1-100 of human DNA topoisomerase I (DNA topoisomerase I (TOP1)) (NP_003277.1).
SequenceMSGDHLHNDSQIEADFRLNDSHKHKDKHKDREHRHKEHKKEKDREKSKHSNSEHKDSEKKHKEKEKTKHKDGSSEKHKDKHKDRDKEKRKEEKVRASGDA
Gene ID7150
Swiss protP11387
SynonymsTOP1; TOPI
Calculated MW90kDa
Observed MW108kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
IHC 1:50 - 1:200
IP 1:50 - 1:100
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Immunoprecipitation    
Positive samplesMCF7, SKOV3, SW480, Jurkat, HeLa, Mouse spleen, Mouse ovary, Mouse testis
Cellular locationNucleus, nucleolus, nucleoplasm
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - DNA topoisomerase I (TOP1) Rabbit pAb (A7741) }

Western blot - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Western blot analysis of extracts of various cell lines, using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - DNA topoisomerase I (TOP1) Rabbit pAb (A7741) }

Immunohistochemistry - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Immunohistochemistry of paraffin-embedded human breast using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at dilution of 1:100 (40x lens).
ABclonal:Immunohistochemistry - DNA topoisomerase I (TOP1) Rabbit pAb (A7741) }

Immunohistochemistry - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Immunohistochemistry of paraffin-embedded human gastric cancer using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at dilution of 1:100 (40x lens).
ABclonal:Immunoprecipitation - DNA topoisomerase I (TOP1) Rabbit pAb (A7741) }

Immunoprecipitation - DNA topoisomerase I (TOP1) Rabbit pAb (A7741)

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741). Western blot was performed from the immunoprecipitate using DNA topoisomerase I (DNA topoisomerase I (TOP1)) antibody (A7741) at a dilition of 1:500.

* For research use only. Not for therapeutic or diagnostic purposes.

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