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DNA topoisomerase II alpha (TOP2A) Rabbit pAb (A16440)

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ABclonal:Western blot - DNA topoisomerase II alpha (TOP2A) Rabbit pAb (A16440)

Western blot analysis of extracts of various cell lines, using DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) antibody (A16440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 300s.

Overview

Product nameDNA topoisomerase II alpha (TOP2A) Rabbit pAb
Catalog No.A16440
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromosome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia.
ImmunogenA synthetic peptide corresponding to a sequence within amino acids 1050-1150 of human DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) (NP_001058.2).
SequenceNQARFILEKIDGKIIIENKPKKELIKVLIQRGYDSDPVKAWKEAQQKVPDEEENEESDNEKETEKSDSVTDSGPTFNYLLDMPLWYLTKEKKDELCRLRNE
Gene ID7153
Swiss protP11388
SynonymsTOP2A; TOP2; TP2A
Calculated MW174kDa/177kDa/178kDa/182kDa
Observed MW182kDa
ReactivityHuman
Tested applicationsWBIHCICCIFIPChIPChIP-seqRIPFCELISAMeDIPNucleotide ArrayDBFACSCoIP
Recommended dilutionWB 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application keyWestern blotting    
Positive samplesHeLa, SKOV3
Cellular locationCytoplasm, Nucleus, nucleoplasm
Customer validation

WB(Homo sapiens)

ABclonal:Western blot - DNA topoisomerase II alpha (TOP2A) Rabbit pAb (A16440) }

Western blot - DNA topoisomerase II alpha (TOP2A) Rabbit pAb (A16440)

Western blot analysis of extracts of various cell lines, using DNA topoisomerase II alpha (DNA topoisomerase II alpha (TOP2A)) antibody (A16440) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 300s.

* For research use only. Not for therapeutic or diagnostic purposes.

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