Product Type > Antibodies > Primary Antibodies > Polyclonal Antibodies

DMP1 Rabbit pAb (A16832)

Publications (7) Datasheet SDS COA

Tested applications: WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition

Reactivity:Human, Mouse, Rat

ABclonal:Western blot - DMP1 Rabbit pAb (A16832)

Western blot analysis of Mouse testis, using DMP1 antibody (A16832) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Overview

Product name DMP1 Rabbit pAb
Catalog No. A16832
Host species Rabbit
Purification method Affinity purification
Isotype IgG

Background

Dentin matrix acidic phosphoprotein is an extracellular matrix protein and a member of the small integrin binding ligand N-linked glycoprotein family. This protein, which is critical for proper mineralization of bone and dentin, is present in diverse cells of bone and tooth tissues. The protein contains a large number of acidic domains, multiple phosphorylation sites, a functional arg-gly-asp cell attachment sequence, and a DNA binding domain. In undifferentiated osteoblasts it is primarily a nuclear protein that regulates the expression of osteoblast-specific genes. During osteoblast maturation the protein becomes phosphorylated and is exported to the extracellular matrix, where it orchestrates mineralized matrix formation. Mutations in the gene are known to cause autosomal recessive hypophosphatemia, a disease that manifests as rickets and osteomalacia. The gene structure is conserved in mammals. Two transcript variants encoding different isoforms have been described for this gene.

Immunogen information

Immunogen A synthetic peptide corresponding to a sequence within amino acids 1-100 of human DMP1 (NP_004398.1).
Sequence MKISILLMFLWGLSCALPVTRYQNNESEDSEEWKGHLAQAPTPPLESSESSEGSKVSSEEQANEDPSDSTQSEEGLGSDDHQYIYRLAGGFSRSTGKGGD
Gene ID 1758
Swiss prot Q13316
Synonyms ARHP; ARHR; DMP-1; DMP1
Calculated MW 56kDa
Observed MW 102kDa

Applications

Reactivity Human, Mouse, Rat
Tested applications WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra) ELISA MeDIP Nucleotide Array DB FACS CoIP FCM CUT&Tag meRIP Inhibition
Recommended dilution
  • WB 1:100 - 1:500
Storage buffer Store at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Application key Western blotting    
Positive samples Mouse testis
Cellular location Cytoplasm, Nucleus, Secreted, extracellular matrix, extracellular space
Customer validation

WB (Homo sapiens, Mus musculus)

WB IHC (Mus musculus)

IF (Mus musculus)

Documents

Certificate of Compliance

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Lot number

Research Area

DMP1 Rabbit pAb images

ABclonal:Western blot - DMP1 Rabbit pAb (A16832)}

Western blot - DMP1 Rabbit pAb (A16832)

Western blot analysis of Mouse testis, using DMP1 antibody (A16832) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

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Related Targets

Based on existing publications, the following genes are closely related to research on DMP1. (Numbers indicate number of published articles where both targets appear.)

1/

Related Research Area

Based on existing publications, the following research topics are related to DMP1. (Distance between topics and target gene indicate popularity.) DMP1

* Data provided by citexs.com, for reference only.

Publishing research using A16832? Please let us know so that we can cite the reference in this datasheet.

Antibodies (1)

ELISA Kits (1)

Secondary Antibodies (23)

* For research use only. Not for therapeutic or diagnostic purposes.

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