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DKK1 Rabbit pAb (A0567)

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ABclonal:Western blot - DKK1 Polyclonal Antibody (A0567)
Review
ABclonal: review for DKK1 Rabbit pAb(A0567)

Overview

Product nameDKK1 Rabbit pAb
Catalog No.A0567
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
ImmunogenRecombinant protein of human DKK1
SequenceEmail for sequence
Gene ID22943
Swiss protO94907
SynonymsDKK1; DKK-1; SK
Calculated MW28kDa
Observed MW36kDa
ReactivityHuman, Mouse
Tested applicationsWBIHCICCIFIPChIPChIPseqRIPFCELISAMeDIPNucleotide Array
Recommended dilutionWB 1:200 - 1:500
IHC 1:50 - 1:100
FC 1:20 - 1:50
Storage bufferStore at 4℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, pH7.3.
Application keyWestern blotting    Immunohistochemistry    Flow Cytometry    
Positive samplesHeLa, Jurkat, U-87MG, MCF7, Mouse heart
Cellular locationSecreted
Customer validation

(ADSCs)

WB(Rattus norvegicus)

ABclonal:Western blot - DKK1 Polyclonal Antibody (A0567) }

Western blot - DKK1 Polyclonal Antibody (A0567)

Western blot analysis of extracts of various cell lines, using DKK1 antibody (A0567) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 30s.

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